FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459345
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24250.5279256332386333No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG19110.41602716912124876No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12250.26668408277003125No Hit
GTGTAAAAGGTGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12150.2645070698494596No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12020.2616769530527164No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC9680.21073485071133896No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9340.20333300678139526No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9320.2028976041972809No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8970.19527805897528No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8640.18809391633739347No Hit
ATTAACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8580.18678770858505045No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8550.18613460470887896No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8410.1830867866200786No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8350.18178057886773558No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT8000.17416103364573468No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7990.1739433323536775No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7850.17089551426487715No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7820.17024241038870566No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG7610.1656706832555051No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7570.16479987808727645No Hit
ATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7520.1637113716269906No Hit
GTTCTATCCCAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7360.16022815095407592No Hit
GTCTAATTTTTAGGCCCATATTAACATTGACATAGCTGACTACTAATTCC6880.14977848893533183No Hit
ACCCATCTCGGGGCCGTTTGGGCCTCTACCGTCCCCTTCTTCATCTGCCG6640.14455365792595978No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6560.14281204758950244No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6550.14259434629744527No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6530.14215894371333093No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6450.1404173333768736No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATCGCTGAGAGTCCAA6390.13911112562453057No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6320.1375872165801304No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6310.13736951528807323No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6280.13671641141190174No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6240.13584560624367306No Hit
TTGACATACTTTCCAATCAATAGGTCGATTTACAGGCAGTTTTCGAAAAC6230.1356279049516159No Hit
CATGTATACAATCAAAGAAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6220.13541020365955872No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5850.12735525585344348No Hit
CATTTGAACCCCAATAAAACTAAACGTTGGGGCTATTCCCTTAACTTCAT5770.12561364551698614No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5610.12213042484407145No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5580.12147732096789994No Hit
ATATTGTACTTAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATCGA5450.11864720417115676No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA5420.11799410029498525No Hit
AAATCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGA5310.1155993860823564No Hit
GGTTGGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5300.11538168479029924No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5210.11342237316178472No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGTGCAGCA5160.11233386670149888No Hit
CAATAAAACTAAACGTTGGGGCTATTCCCTTAACTTCATGGGATATGTAA5150.11211616540944169No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT5130.11168076282532736No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5130.11168076282532736No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG5060.1101568537809272No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5000.10885064602858417No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4990.108632944736527No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4950.10776213956829833No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4840.10536742535566948No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCGAT4780.10406121760332648No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4770.10384351631126931No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAAA4770.10384351631126931No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4710.10253730855892629No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4700.10231960726686913No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4640.10101339951452612No Hit
CTCCAAAAGACACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTAAGA4610.10036029563835461No Hit
GCCTAATCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4600.10014259434629744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)