FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649691
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT28230.43451425369906616No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT27870.42897315800896113No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG22680.34908902847661427No Hit
CTCCAAAAGATACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA19130.29444766819919005No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG18960.2918310396788627No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17280.26597259312503946No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT16150.2485797094310988No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA15480.2382671146745145No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15050.23164858371133354No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13700.21086947487343982No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA13160.20255783133828237No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA13050.20086471876630585No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAAAA12240.1883972534635696No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG12150.18701197954104337No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11980.18439535102071603No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT11730.18054736790258755No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11510.1771611427586345No Hit
CATGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11470.17654546545973393No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT11150.17162004706852949No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC11030.16977301517182783No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11000.16931125719765241No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT10930.16823382192457645No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATT10830.16669462867732507No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGTACAACAGAGT10790.16607895137842452No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10600.1631544842086469No Hit
TGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTT10330.15899866244106814No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC10040.1545350020240391No Hit
ACTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9900.15238013147788718No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG9780.15053309958118552No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9550.14699295511250735No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9520.14653119713833193No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG9500.14622335848888166No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC9220.14191361739657776No Hit
CTTTTACACAATGTGGCTATCCTGCTTTAATGCCTTTATATGCATGTATA9200.1416057787471275No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9190.14145185942240235No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA9140.14068226279877666No Hit
GTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAA9040.13914306955152528No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC8750.13467940913449625No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCA8720.1342176511603208No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA8620.13267845791306945No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC8530.13129318399054318No Hit
GTATAGGCCCCTCACTTTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG8130.12513641100153766No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7990.12298154045538572No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7950.12236586315648515No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTTTGT7930.12205802450703487No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG7740.11913355733725725No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7650.11774828341473101No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC7570.11651692881692989No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7560.11636300949220475No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTTTGTACAATA7550.11620909016747961No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC7550.11620909016747961No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC7500.11543949354385394No Hit
GCTTAGAGTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACAT7490.11528557421912877No Hit
CTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA7380.11359246164715227No Hit
GAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCA7340.11297678434825172No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7320.11266894569880143No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT7290.11220718772462601No Hit
GTTGTCAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7230.11128367177627518No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7180.11051407515264948No Hit
CATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7180.11051407515264948No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT7150.11005231717847407No Hit
ATACAGCACTCAGGCAAGCTATTCTTTGTTGGGGTGAGTTGATGAATCTG7090.10912880123012325No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA7000.107743527307597No Hit
GCATAGACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTT7000.107743527307597No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6980.10743568865814672No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6940.10682001135924617No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6910.10635825338507075No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTTTGTACAAT6890.10605041473562048No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTATGCCAAGTGTTTGCTG6810.10481906013781936No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6800.10466514081309422No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6790.10451122148836908No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6780.10435730216364394No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC6760.10404946351419367No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6760.10404946351419367No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6750.10389554418946854No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGC6740.1037416248647434No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6710.10327986689056796No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6700.10312594756584283No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6700.10312594756584283No Hit
GAGTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGT6680.10281810891639255No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6680.10281810891639255No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATTTACAT6670.10266418959166743No Hit
GTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCCTG6620.10189459296804174No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6600.10158675431859146No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAAGAACATATTGTACAAAAA6600.10158675431859146No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC6520.10035539972079034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)