FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649691
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG27130.41758312797930097No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT24850.38248952194196933No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT21240.32692464571619434No Hit
CTCCAAAAGATACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA18290.28151844492227845No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17120.2635098839294372No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16080.2475022741580228No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT15430.23749751805088878No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA13080.20132647674048124No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12130.1867041408915931No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA12060.1856267056185171No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11990.18454927034544114No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11520.17731506208335965No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC11310.17408275626413172No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA11140.17146612774380435No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11120.1711582890943541No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAAAA10940.1683877412493016No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT10830.16669462867732507No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT10540.16223096826029604No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG10500.16161529096139549No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10270.15807514649271728No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9250.14237537537075318No Hit
CATGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9240.14222145604602804No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC9180.14129794009767718No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATT8770.13498724778394652No Hit
CTTTTACACAATGTGGCTATCCTGCTTTAATGCCTTTATATGCATGTATA8770.13498724778394652No Hit
GTGTATACATGCATATAAAGGCATTAAAGCAGGATAGCCACATTGTGTAA8650.13314021588724487No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC8610.13252453858834431No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8490.13067750669164263No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTTTGTACAATA8170.12575208830043821No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG8030.12359721775428627No Hit
GAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCA7980.12282762113066058No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGTACAACAGAGT7810.12021099261033323No Hit
TGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTT7720.11882571868780697No Hit
ACTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7710.11867179936308184No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA7690.11836396071363156No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7610.11713260611583044No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC7560.11636300949220475No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7430.11436205827077796No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7360.11328462299770199No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7270.11189934907517574No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCA7240.11143759110100032No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7070.10882096258067298No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7030.10820528528177242No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA6990.10758960798287186No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6840.10528081811199477No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6830.10512689878726963No Hit
GTATAGGCCCCTCACTTTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG6790.10451122148836908No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6730.10358770554001827No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6730.10358770554001827No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6730.10358770554001827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)