FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492866
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT22020.44677457970320533No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18250.3702832007076974No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT16930.3435010733140448No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTCAATTTCAT16250.32970421980822373No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA15980.3242260573867948No Hit
TGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA15280.310023414071979No Hit
CCTCTTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15170.30779157012250796No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14920.30271919751007376No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14580.2958207707571632No Hit
TCTGCGGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCTATTCG13030.2643720605600711No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC12570.25503889495319215No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG12440.2524012611947264No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12250.24854625800927638No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT11380.2308944013180053No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA11210.22744518794155003No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATGT10900.22115544590213163No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTACCAATTACATATCCCATGAA10890.22095255099763422No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA10850.22014097137964478No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG10370.2104020159637711No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA10320.20938754144128424No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTGTGTACAATATGTT10290.20877885672779214No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCA9660.19599647774445794No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA9600.19477910831747372No Hit
GTTGATAGGTGTAAGCAATTTGTAGGACCCCTAACAGTAAATGAAATCAG9500.19275015927250003No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT9450.1917356847500132No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9320.1890980509915474No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8920.18098225481165264No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8880.18017067519366317No Hit
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT8700.17651856691271056No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCATG8650.17550409239022371No Hit
CCTATCAACAATGTCTTCATGCAAATGAATGTCAGGAAAAGAAGGAGTTT8370.16982303506429738No Hit
GCTTTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTT8310.1686056656373132No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8120.16475066245186318No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7910.16048986945741844No Hit
CCTGATTTCATTTACTGTTAGGGGTCCTACAAATTGCTTACACCTATCAA7810.15846092041244478No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTGTGT7720.15663486627196843No Hit
GGTGTAAGCAATTTGTAGGACCCCTAACAGTAAATGAAATCAGGAGACTA7690.15602618155847633No Hit
ACCAATTACATATCCCATGAAATTGAGGGAATATCCCCATCTTTTTGTTT7640.1550117070359895No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG7570.15359144270450792No Hit
TCTGTGGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCTATTCG7250.14709880576059212No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG7240.14689591085609477No Hit
GCTTACACCTATCAACAATGTCTTCATGCAAATGAATGTCAGGAAAAGAA7150.14506985671561845No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCG7130.1446640669066237No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7110.14425827709762898No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCA7000.14202643314815794No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6970.1414177484346658No Hit
GGAACATATTGTACACAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA6890.13979458919868687No Hit
GGGTAAAGGTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA6880.1395916942941895No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTAAC6710.13614248091773423No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6660.13512800639524739No Hit
CCTCTTGCATATAGACCACCAAATATTCAAGAACAGTTTCTCTTCCAAAA6470.13127300320979737No Hit
GATTAACTACATGCTCGGGATAATAGGGTTTGATTCCTTTATCTAAGGGC6460.13107010830530003No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGATAGCACGACAGAAT6450.13086721340080265No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG6440.13066431849630528No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC6400.1298527388783158No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCG6360.12904115926032633No Hit
GTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6150.12478036626588161No Hit
CTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCT6140.12457747136138424No Hit
ACCCCTAACAGTAAATGAAATCAGGAGACTAAAATTAATTATGCCTGCTA6140.12457747136138424No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6130.12437457645688686No Hit
GTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCA6100.12376589174339477No Hit
GTACAATATGTTCCTGTGGCAAGGAACCCCAACTACCAATTACATATCCC6090.12356299683889739No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC6040.12254852231641053No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5930.12031667836693949No Hit
GTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAA5850.11869351913096055No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5840.11849062422646318No Hit
CAGTAAATGAAATCAGGAGACTAAAATTAATTATGCCTGCTAGATTTTAT5680.11524430575450528No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5680.11524430575450528No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAACGGCACTAGTAAACTGAG5520.1119979872825474No Hit
GAAATCAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATGT5470.11098351276006055No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5460.11078061785556317No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCG5440.11037482804656844No Hit
CCCTAACAGTAAATGAAATCAGGAGACTAAAATTAATTATGCCTGCTAGA5340.10834587900159474No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCGCGCATGCGCTGATGGCC5290.10733140447910791No Hit
CTGTTAGGGGTCCTACAAATTGCTTACACCTATCAACAATGTCTTCATGC5280.10712850957461054No Hit
TTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTT5270.10692561467011318No Hit
CTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT5270.10692561467011318No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5250.10651982486111844No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAAT5230.10611403505212369No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAA5170.1048966656251395No Hit
AGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCATTACT5160.10469377072064212No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAA5130.10408508600715002No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT5100.10347640129365791No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5090.10327350638916055No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5070.1028677165801658No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG5050.10246192677117107No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5030.10205613696217632No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5010.1016503471531816No Hit
TTACATATCCCATGAAATTGAGGGAATATCCCCATCTTTTTGTTTTGTGA5010.1016503471531816No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4990.10124455734418684No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC4980.1010416624396895No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4970.10083876753519212No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4940.10023008282170003No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)