FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences492866
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT20660.4191808726915632No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18040.36602240771325273No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT16670.3382258057971132No Hit
CCTCTTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15050.3053568312685395No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTCAATTTCAT14770.2996757739426132No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG14630.29683524527965005No Hit
TGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA14220.28851655419525796No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA14150.28709628986377633No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC13670.27735733444790267No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13000.263763375846579No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11620.23576387902594212No Hit
TCTGCGGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCTATTCG11330.22987992679551847No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10520.21344543953123163No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA10360.21019912105927374No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA10330.2095904363457816No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGATTTTATCCCAATGT9950.2018804299748816No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTACCAATTACATATCCCATGAA9860.2000543758344053No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTGTGTACAATATGTT9770.19822832169392898No Hit
GTTGATAGGTGTAAGCAATTTGTAGGACCCCTAACAGTAAATGAAATCAG9680.19640226755345264No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG9270.18808357646906054No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9020.18301120385662636No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCATG8960.18179383442964214No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8730.17712725162620266No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8560.1736780382497474No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA8530.17306935353625527No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG8460.1716490892047737No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7860.15947539493493162No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7800.1582580255079474No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG7740.15704065608096318No Hit
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT7730.1568377611764658No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7720.15663486627196843No Hit
CCTATCAACAATGTCTTCATGCAAATGAATGTCAGGAAAAGAAGGAGTTT7720.15663486627196843No Hit
GCTTTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTT7670.15562039174948158No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7660.15541749684498424No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCA7600.15420012741800002No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT7530.15277986308651845No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA7370.14953354461456056No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7300.14811328028307896No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA7290.1479103853785816No Hit
GGAACATATTGTACACAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA7160.14527275162011583No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA7160.14527275162011583No Hit
CCTGATTTCATTTACTGTTAGGGGTCCTACAAATTGCTTACACCTATCAA6970.1414177484346658No Hit
GCTTACACCTATCAACAATGTCTTCATGCAAATGAATGTCAGGAAAAGAA6870.13938879938969212No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGACAGTG6710.13614248091773423No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCG6500.1318816879232895No Hit
GGTGTAAGCAATTTGTAGGACCCCTAACAGTAAATGAAATCAGGAGACTA6480.13147589811429475No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTGTGT6310.12802668473783949No Hit
GTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAA6290.12762089492884476No Hit
GTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCA6250.1268093153108553No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCA6220.1262006305973632No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6050.1227514172209079No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTAAC6010.12193983760291843No Hit
GATTAACTACATGCTCGGGATAATAGGGTTTGATTCCTTTATCTAAGGGC5960.12092536308043159No Hit
TCTGTGGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCTATTCG5850.11869351913096055No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGATAGCACGACAGAAT5760.11686746499048424No Hit
GTACAATATGTTCCTGTGGCAAGGAACCCCAACTACCAATTACATATCCC5680.11524430575450528No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC5670.11504141085000791No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5670.11504141085000791No Hit
ACCAATTACATATCCCATGAAATTGAGGGAATATCCCCATCTTTTTGTTT5660.11483851594551053No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5640.11443272613651581No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAA5620.11402693632752106No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5600.11362114651852634No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5560.11280956690053687No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5500.11159219747355265No Hit
GTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5490.11138930256905528No Hit
GGGTAAAGGTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA5450.11057772295106581No Hit
TTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTT5410.10976614333307634No Hit
CGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5410.10976614333307634No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCG5390.10936035352408159No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT5380.10915745861958422No Hit
CTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCT5350.10854877390609212No Hit
CCTCTTGCATATAGACCACCAAATATTCAAGAACAGTTTCTCTTCCAAAA5270.10692561467011318No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA5250.10651982486111844No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5240.10631692995662106No Hit
ATGTAATTGGTAGTTGGGGTTCCTTGCCACAGGAACATATTGTACACAAA5220.10591114014762634No Hit
CCCTAACAGTAAATGAAATCAGGAGACTAAAATTAATTATGCCTGCTAGA5190.10530245543413422No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5180.10509956052963687No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCGCGCATGCGCTGATGGCC5100.10347640129365791No Hit
CAGTAAATGAAATCAGGAGACTAAAATTAATTATGCCTGCTAGATTTTAT5100.10347640129365791No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5090.10327350638916055No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5070.1028677165801658No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5030.10205613696217632No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5010.1016503471531816No Hit
GCTCAGAATACTGCCTCTGCCATATCATCAACCTTCTCGAAGACTGGGGA5010.1016503471531816No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATAGAAGGAAA5000.10144745224868422No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4950.10043297772619737No Hit
CTGTTAGGGGTCCTACAAATTGCTTACACCTATCAACAATGTCTTCATGC4930.10002718791720265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)