FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403077
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18040.44755716649672395No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12470.3093701699675248No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12240.3036640641862473No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11090.2751335352798597No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT10590.2627289574944738No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTACCATT10090.2503243797090879No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9730.24139308370360996No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9390.23295797080954755No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9090.22551522413831598No Hit
GCCTAGCAGCCATGGAAAAGATGTATACTTGCGGGAGAGCACGACAGAAT8900.22080148457986937No Hit
ATTTAGTACCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTC8800.21832056902279218No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8610.2136068294643455No Hit
ACTCACAACTGTGCCAGCAACTCCTCCTCCTGCCTCCACCAATCGGCAGT8610.2136068294643455No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA8010.19872133612188242No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7910.19624042056480526No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA7650.18979004011640457No Hit
GTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAG7520.18656484989220423No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7490.18582057522508105No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA7340.18209920188946527No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7230.17937019477668037No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7180.1781297369981418No Hit
ATATGTTCCTGCGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7030.17440836366252602No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6590.1634923352113864No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6490.16101141965430923No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6320.15679386320727803No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6300.1562976800958626No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCCACTTACAAGGCC6180.15332058142736996No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6130.1520801236488314No Hit
CTTACAAGGCCTTCCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT5980.1483587503132156No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5890.14612592631184612No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT5860.14538165164472297No Hit
GTTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5810.14414119386618438No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5780.14339691919906122No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5740.14240455297623036No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5630.13967554586344544No Hit
ATTCTGAACAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5530.13719463030636828No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5480.13595417252782968No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5400.13396944008216793No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5400.13396944008216793No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCGGT5240.12999997519084444No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5230.1297518836351367No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5210.12925570052372126No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5200.12900760896801355No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5070.1257824187438132No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5000.12404577785385919No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4960.12305341163102831No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4940.12255722851961287No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4920.12206104540819745No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4900.12156486229678201No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4880.12106867918536657No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4870.12082058762965885No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4860.12057249607395114No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4760.11809158051687395No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4750.11784348896116623No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4700.11660303118262763No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4700.11660303118262763No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTCATTCCAGGATCATCAACAACC4670.11585875651550447No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4590.11387402406984273No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4550.11288165784701185No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG4550.11288165784701185No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4530.11238547473559643No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATATCCTCCTGC4530.11238547473559643No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4520.11213738317988871No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCGCAG4510.11188929162418099No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4510.11188929162418099No Hit
GACTTCTTTCCTTCTATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTA4450.11040074228993468No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCGCAGGAACATATTGTACAAAAA4420.10965646762281152No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4400.10916028451139609No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4300.1066793689543189No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4270.10593509428719575No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4270.10593509428719575No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4250.1054389111757803No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4230.10494272806436486No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAC4200.10419845339724172No Hit
GGATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATG4190.103950361841534No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC4170.10345417873011856No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTC4150.10295799561870313No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG4140.1027099040629954No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTC4130.10246181250728768No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGGAAGGCCTTGTAAGTGGGCGA4120.10221372095157996No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA4110.10196562939587224No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC4060.10072517161733366No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4060.10072517161733366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)