FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403077
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16130.4001716793565498No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12560.3116029939688943No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11890.29498085973647714No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT10720.2659541477186741No Hit
ATTTAGTACCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTC9320.23122132991959354No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTACCATT9240.22923659747393177No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8930.22154575924699252No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8790.21807247746708447No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA8750.21708011124425358No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8600.2133587379086378No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8470.21013354768443746No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7650.18979004011640457No Hit
GTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAG7540.18706103300361965No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7480.18557248366937334No Hit
ACTCACAACTGTGCCAGCAACTCCTCCTCCTGCCTCCACCAATCGGCAGT6980.17316790588398742No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6810.16895034943695622No Hit
GCCTAGCAGCCATGGAAAAGATGTATACTTGCGGGAGAGCACGACAGAAT6760.16770989165841763No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA6610.16398851832280184No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6590.1634923352113864No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6500.16125951121001694No Hit
ATATGTTCCTGCGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6430.15952287032006293No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6360.1577862294301089No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6350.15753813787440116No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6260.1553053138730317No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6160.1528243983159545No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6090.1510877574260005No Hit
GTTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6080.15083966587029277No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6050.15009539120316961No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA6010.14910302498033873No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5930.147118292534677No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5700.14141218675339948No Hit
CTTACAAGGCCTTCCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT5530.13719463030636828No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5480.13595417252782968No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5240.12999997519084444No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5170.1282633343008904No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5150.12776715118947496No Hit
ATTCTGAACAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5050.1252862356323978No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT5050.1252862356323978No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4930.12230913696390515No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4930.12230913696390515No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4870.12082058762965885No Hit
GACTTCTTTCCTTCTATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTA4840.1200763129625357No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4830.11982822140682797No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4720.11709921429404307No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCGGT4700.11660303118262763No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCGCAGGAACATATTGTACAAAAA4680.11610684807121219No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4600.11412211562555047No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4550.11288165784701185No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4510.11188929162418099No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGGAAGGCCTTGTAAGTGGGCGA4510.11188929162418099No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4500.11164120006847326No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4460.1106488338456424No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCCACTTACAAGGCC4450.11040074228993468No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTCATTCCAGGATCATCAACAACC4440.11015265073422696No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4440.11015265073422696No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCGCAG4400.10916028451139609No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4380.10866410139998064No Hit
GGATATTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATG4370.10841600984427292No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4370.10841600984427292No Hit
GTATACATCTTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4320.10717555206573434No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATATCCTCCTGC4300.1066793689543189No Hit
GTTTTATCCCAATGGTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4280.10618318584290347No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4230.10494272806436486No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4220.10469463650865717No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4180.10370227028582628No Hit
GTTCAGATACTGTTTACTTAGGAAGGCCTTGTAAGTGGGCGAGAAAGTAA4180.10370227028582628No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC4170.10345417873011856No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4140.1027099040629954No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4080.1012213547287491No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4050.10047708006162594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)