FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407754
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA16430.40293902696233513No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG14910.36566164893538755No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA14680.36002099304973095No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATAAAACCTTAT12800.3139147623321905No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12380.3036144341931655No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11380.2790898433859631No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAGCCCATGAA11250.2759016465810268No Hit
ATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA10970.2690347611550101No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC10550.25873443301598514No Hit
CCCTTAACTTCATGGGCTATGTAATTGGAAGTTGGGGTACTTTACCACAG10210.2503960721415363No Hit
GCACTGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG10190.24990558032539226No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10190.24990558032539226No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9400.23053115358770238No Hit
CATGTATACAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9180.22513574361011784No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8840.21679738273566906No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8620.21140197275808453No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8100.1986491855383393No Hit
CAATAAAACTAAGCGTTGGGGCTACTCCCTTAACTTCATGGGCTATGTAA8050.19742295599797918No Hit
GATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA7910.19398951328497083No Hit
GTTATTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTC7640.1873678737670262No Hit
GTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTGCATGTTCAGG7410.18172721788136964No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7310.17927475880064941No Hit
TTGTTCACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAG6960.1706911520181286No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA6950.17044590611005656No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6740.16529574204054406No Hit
CATTTGAACCCCAATAAAACTAAGCGTTGGGGCTACTCCCTTAACTTCAT6660.16333377477596786No Hit
GCTTAGACTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT6570.16112656160331965No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6500.15940984024681548No Hit
GGAACATATTGTACTAAAAATTAAGCAATGTTTTCGAAAACTGCCTGTAA6440.15793836479838336No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6440.15793836479838336No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6190.15180721709658276No Hit
GTTTTATTGGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6110.14984524983200656No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6100.14960000392393452No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTC6040.1481285284755024No Hit
GGATAATAAGGTTTTATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT5920.1451855775786381No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC5910.14494033167056608No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAAC5840.1432236103140619No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5820.14273311849791787No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5690.13954492169298155No Hit
AGTTAAGGGAGTAGCCCCAACGCTTAGTTTTATTGGGGTTCAAATGTATA5680.1392996757849095No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5480.13439475762346906No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5470.13414951171539705No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5460.133904265807325No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTT5450.133659019899253No Hit
CCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5390.13218754445082084No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5390.13218754445082084No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATAAAAC5390.13218754445082084No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTAAAA5380.1319422985427488No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATA5320.13047082309431665No Hit
GATTTAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCT5310.13022557718624467No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT5300.12998033127817263No Hit
TTATTAATAGATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5130.1258111508409482No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5080.1245849213005881No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5080.1245849213005881No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5050.12384918357637204No Hit
TTCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5050.12384918357637204No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5020.12311344585215596No Hit
ACAGCAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA5000.12262295403601192No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC4990.1223777081279399No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG4940.12115147858757977No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4940.12115147858757977No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4810.11796328178264345No Hit
CTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTC4790.11747278996649942No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4760.11673705224228334No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4750.1164918063342113No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4710.11551082270192321No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4690.11502033088577918No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4690.11502033088577918No Hit
GTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAG4660.1142845931615631No Hit
GGGAATTAGTAGTCGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGA4640.11379410134541906No Hit
GATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA4630.11354885543734704No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAAA4600.11281311771313095No Hit
GTTCACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAG4590.11256787180505892No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATAGCCCATGAAGTTAAGGGAG4560.11183213408084286No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4520.11085115044855477No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTCAC4500.11036065863241071No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4480.10987016681626668No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG4450.1091344290920506No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4430.10864393727590656No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4410.1081534454597625No Hit
CTATCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCT4370.10717246182747442No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4320.10594623228711429No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4320.10594623228711429No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4270.10472000274675418No Hit
GGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAGACGGGGA4260.10447475683868215No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4250.10422951093061013No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCA4220.10349377320639407No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTTAGTACAATA4190.10275803548217798No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4190.10275803548217798No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4180.10251278957410595No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCATCTATTCG4180.10251278957410595No Hit
GCTTTGGGGCATGGACATTGACACCTATAAAGAATTTGGAGCTTCTGTGG4160.10202229775796191No Hit
GGATGGGGCTTGGCCATTGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4150.10177705184988989No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG4110.1007960682176018No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4110.1007960682176018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)