FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407754
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG16660.4085796828479917No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA16500.4046557483188393No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11810.28963541743306015No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATAAAACCTTAT11140.27320394159223454No Hit
ATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA11030.27050623660344225No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATAGCCCATGAA10850.2660918102581458No Hit
CCCTTAACTTCATGGGCTATGTAATTGGAAGTTGGGGTACTTTACCACAG10300.2526032853141845No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10150.24892459669310418No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA9960.24426492443973571No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9950.24401967853166373No Hit
GCACTGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9570.2347003340249268No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9200.2256262354262619No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8790.21557115319530895No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8100.1986491855383393No Hit
GATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA7970.195460988733403No Hit
CATGTATACAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7870.19300852965268275No Hit
GTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTGCATGTTCAGG7740.18982033284774644No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7730.18957508693967443No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7630.18712262785895417No Hit
CAATAAAACTAAGCGTTGGGGCTACTCCCTTAACTTCATGGGCTATGTAA7280.17853902107643335No Hit
TTGTTCACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAG7190.1763318079037851No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7020.17216262746656072No Hit
CTCCAAAAGACACCAAATACTCTAGAACAGTTTCTCTTCCAAAAGTAAGA6960.1706911520181286No Hit
GCTTAGACTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT6810.16701246339704823No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG6670.16357902068403987No Hit
GTTATTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTC6570.16112656160331965No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6530.16014557797103154No Hit
TTCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6500.15940984024681548No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6420.15744787298223928No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6420.15744787298223928No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6280.15401443026923095No Hit
GGATAATAAGGTTTTATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6120.15009049574007857No Hit
GTTTTATTGGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT6120.15009049574007857No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5920.1451855775786381No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTC5870.14395934803827798No Hit
CATTTGAACCCCAATAAAACTAAGCGTTGGGGCTACTCCCTTAACTTCAT5830.1429783644059899No Hit
GGAACATATTGTACTAAAAATTAAGCAATGTTTTCGAAAACTGCCTGTAA5810.14248787258984583No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5800.14224262668177381No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5750.1410163971414137No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAAC5650.13856393806069345No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5530.13562098716382917No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATAAAAC5400.13243279035889285No Hit
GTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAG5360.13145180672660478No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5270.12924459355395654No Hit
AGTTAAGGGAGTAGCCCCAACGCTTAGTTTTATTGGGGTTCAAATGTATA5240.12850885582974048No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAATTTTTAGTACAATA5240.12850885582974048No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5210.1277731181055244No Hit
GTTCACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAG5180.12703738038130832No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5170.1267921344732363No Hit
GATTTAGACCTTCGTCTGCGAGGCGAGGGAGTTCTTCTTCTAGGGGACCT5150.12630164265709226No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTGCACTTCGCTTCAC5130.1258111508409482No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5120.1255659049328762No Hit
CTATCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCT5110.12532065902480416No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5050.12384918357637204No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC5030.12335869176022798No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT5010.12286819994408392No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4920.12066098677143572No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4790.11747278996649942No Hit
GTTCAGGATAATAAGGTTTTATGCCTTTATCCAAGGGCAAATATTTGGTA4780.11722754405842739No Hit
GGGAATTAGTAGTCGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGA4770.11698229815035537No Hit
TTATTAATAGATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4770.11698229815035537No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4760.11673705224228334No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC4720.11575606860999524No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4670.11452983906963513No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTAAAA4670.11452983906963513No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATA4600.11281311771313095No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4520.11085115044855477No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4520.11085115044855477No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC4490.11011541272433868No Hit
GGATGGGGCTTGGCCATTGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4470.10962492090819465No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4390.10766295364361844No Hit
GTCTTTGGGTATACATTTGAACCCCAATAAAACTAAGCGTTGGGGCTACT4380.10741770773554644No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4330.1061914781951863No Hit
CTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTC4330.1061914781951863No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCCAATAAA4310.10570098637904227No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4300.10545574047097024No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCA4290.10521049456289822No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTT4290.10521049456289822No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4270.10472000274675418No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4270.10472000274675418No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG4250.10422951093061013No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCATCTATTCG4210.10324852729832203No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATAGCCCATGAAGTTAAGGGAG4150.10177705184988989No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCA4150.10177705184988989No Hit
ACAGCAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA4110.1007960682176018No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4100.10055082230952976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)