FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507616
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG52381.0318823677740654No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT33150.6530527012544916No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT29620.58351194603795No Hit
CTCTTACAGTCAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG22260.4385204564079935No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT21580.42512450356174747No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA16400.3230788627624031No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC15420.30377293071928385No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTCAATGAAAAA15370.30278793418647165No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA14430.28426999936960223No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT13840.2726470402824182No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12760.2513711151736746No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA12630.24881012418836285No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT12480.24585513458992625No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12190.24014215469961545No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12050.23738416440774127No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC11690.2302921893714934No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG11670.2298981907583685No Hit
CTTACAGTCAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT11340.22339721364180798No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC11300.22260921641555823No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTCTCTCTTCCAAAAGTAAGA10940.21551724137931033No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCTGT10780.2123652524743113No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10690.21059225871524934No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10480.20645527327743807No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC9780.1926653218180672No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9690.19089232805900525No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA9410.1853763474752569No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9370.18458835024900713No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9330.18380035302275735No Hit
CTATTAATAATGTCTTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9300.18320935510307004No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTGAC9160.18045136481119586No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9120.1796633675849461No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8970.17670837798650949No Hit
CTCAAGAACAGTCTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA8890.17513238353400995No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG8850.1743443863077602No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8810.17355638908151041No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA8470.16685841265838744No Hit
ACCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGC8140.1603574355418269No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7950.15661444871714053No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7790.15346245981214146No Hit
CATGTATACAATCTAGGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7660.15090146882682973No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC7550.14873447645464288No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT7410.1459764861627687No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7380.1453854882430814No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA7310.1440064930971443No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7140.1406575048855828No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7120.14026350627245793No Hit
CAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTT7070.1392785097396457No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7020.1382935132068335No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6840.13474752568870957No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6780.13356552984933492No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTCAATGAAA6760.13317153123621003No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6730.13258053331652273No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6690.13179253609027294No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6680.1315955367837105No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6640.13080753955746074No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAAT6500.1280495492655866No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATATGTT6500.1280495492655866No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6500.1280495492655866No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6470.12745855134589928No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTCAATGAAAAAAG6380.1256855575868373No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG6360.1252915589737124No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT6280.12371556452121289No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6200.12213957006871337No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTCAATGAAAAAA6180.12174557145558848No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6090.11997257769652651No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6050.11918458047027675No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAAT5960.11741158671121478No Hit
GGGTAAAGGTGCAGGTATTGTTGATACAGAAAGGCCTTGTAAGTTGGCGA5950.11721458740465233No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5920.11662358948496501No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG5900.11622959087184014No Hit
CAATATGTGGGCCCTCTTACAGTCAATGAAAAAAGGAGATTAAAATTAAT5900.11622959087184014No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5900.11622959087184014No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT5830.11485059572590305No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5800.11425959780621572No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG5740.11307760196684108No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA5700.11228960474059131No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5670.11169860682090399No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5660.11150160751434154No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5640.11110760890121667No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5630.11091060959465422No Hit
CATTTACAGGAAGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5510.10854661791590493No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5440.10716762276996784No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5430.10697062346340541No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5410.10657662485028052No Hit
TAATAGATGTCAACAATATGTGGGCCCTCTTACAGTCAATGAAAAAAGGA5400.10637962554371809No Hit
GCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCAT5290.10421263317153123No Hit
CCCTCTTACAGTCAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTA5280.10401563386496879No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5280.10401563386496879No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5250.10342463594528147No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5240.10322763663871903No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC5220.10283363802559414No Hit
GACTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCT5210.1026366387190317No Hit
GAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCT5170.10184864149278194No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5170.10184864149278194No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC5120.10086364495996973No Hit
GAATATGGTGACCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTCTTATA5090.10027264704028241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)