FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441040
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18210.41288771993469975No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT15440.3500816252494105No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14660.33239615454380556No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12700.28795574097587523No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12490.28319426809359693No Hit
ATGTCATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTGAAA11570.2623344821331398No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAACCCCAACGTTTGGTTTTA10680.2421549065844368No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10450.23693996009432253No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9940.22537638309450392No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9830.22288227825140575No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9790.22197533103573372No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9390.21290585887901325No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8820.19998186105568655No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8780.1990749138400145No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC8690.19703428260475242No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8260.1872846000362779No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7980.18093596952657356No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7730.17526754942862327No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7490.16982586613459097No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTGAAA7480.16959912933067295No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7440.1686921821150009No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT7350.1666515508797388No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7350.1666515508797388No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7320.16597134046798478No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7270.1648376564483947No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6930.15712860511518228No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6900.15644839470342825No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6630.15032650099764194No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6570.14896608017413387No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6540.1482858697623798No Hit
ATTCAAGATCTTCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA6330.14352439688010157No Hit
ATCTCAATGTTAGTATCCCGTGGACTCATAAGGTGGGAAACTTTACTGGG6300.14284418646834754No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6280.14239071286051153No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6240.14148376564483947No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGGGGAAAGGAGGGAGTTTG6220.14103029203700346No Hit
ATCTCGAATAGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGAGTAACTCCA6080.13785597678215128No Hit
CTTTTATATAGAATGCCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT5940.1346816615272991No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5790.13128060946852893No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT5730.12992018864502086No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5680.1287865046254308No Hit
CTTTAGGGCATGGACATTGACCTGTATAAAGAATTTGGAGCTTCTGTGGA5670.1285597678215128No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5650.12810629421367675No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5650.12810629421367675No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5620.12742608380192272No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5520.1251587157627426No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5490.12447850535098857No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5480.12425176854707057No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5440.1233448213313985No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCAATACAATA5410.12266461091964447No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5240.11881008525303828No Hit
GCAGACGAAGAAGGGGACGGTAGAGGCCCAAACGGCCCCGAGACGGGTCG4940.1120079811354979No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT4890.11087429711590785No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4870.11042082350807184No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4830.1095138762923998No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4790.10860692907672773No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4740.10747324505713768No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4690.1063395610375476No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCAATACAATATGTT4680.1061128242336296No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4630.10497914021403955No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4620.10475240341012153No Hit
GTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA4620.10475240341012153No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4620.10475240341012153No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4610.10452566660620352No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4610.10452566660620352No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4560.10339198258661345No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC4540.10293850897877743No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4510.1022582985670234No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTA4500.10203156176310539No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4460.10112461454743335No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTG4440.10067114093959732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)