FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441040
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16450.3729820424451297No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14660.33239615454380556No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13780.3124433157990205No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13670.30994921095592237No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11670.26460185017231996No Hit
ATGTCATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTGAAA10890.24691637946671505No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10690.2423816433883548No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10450.23693996009432253No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10130.22968438236894612No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC9860.22356248866315978No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9670.21925448938871758No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9130.20701070197714494No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAACCCCAACGTTTGGTTTTA8870.2011155450752766No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8760.19862144023217848No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8350.18932523127154No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8110.18388354797750772No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7940.18002902231090148No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7260.1646109196444767No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT7180.16279702521313258No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTGAAA6950.15758207872301833No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6940.1573553419191003No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6930.15712860511518228No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6890.15622165789951026No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6770.1535008162524941No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6520.1478323961545438No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCAATACAATA6440.1460185017231997No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGGGGAAAGGAGGGAGTTTG6390.14488481770360964No Hit
CTTTTATATAGAATGCCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT6350.14397787048793761No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6310.14307092327226553No Hit
ATTCAAGATCTTCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA6180.1401233448213314No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6140.13921639760565935No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6090.1380827135860693No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6020.1364955559586432No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5870.13309450389987304No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5810.13173408307636494No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5790.13128060946852893No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5690.1290132414293488No Hit
ATCTCAATGTTAGTATCCCGTGGACTCATAAGGTGGGAAACTTTACTGGG5530.12538545256666062No Hit
ATCTCGAATAGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGAGTAACTCCA5490.12447850535098857No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5270.1194902956647923No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5250.11903682205695629No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5180.1174496644295302No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT5080.11518229639035009No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5020.113821875566842No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4980.11291492835116997No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4920.11155450752766188No Hit
CTTTAGGGCATGGACATTGACCTGTATAAAGAATTTGGAGCTTCTGTGGA4910.11132777072374388No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4910.11132777072374388No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4790.10860692907672773No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4770.10815345546889171No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4770.10815345546889171No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4700.10656629784146564No Hit
ATTTACATACTTTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA4690.1063395610375476No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4570.10361871939053148No Hit
CTCCATGTTCGGTGCAGGGTCCCCAATCCTCGAGAAGATTGACGATATGG4550.10316524578269544No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTCAATACAATATGTT4540.10293850897877743No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4520.1024850353709414No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4510.1022582985670234No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4490.10180482495918738No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4480.10157808815526936No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4460.10112461454743335No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT4430.10044440413567929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)