FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435034
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19500.4482408271537397No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG14610.33583581972903265No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA13380.3075621675547199No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGAATTAAACCCTAT12410.2852650597424569No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG12360.2841157242882166No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA12240.2813573191980397No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11790.27101330010987645No Hit
CCCTAATAAAACCAAACGATGGGGCTACTCCCTTAACTTCATGGGATATG10810.24848632520676545No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACACACT10780.24779672393422125No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT9890.22733855284874285No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9800.22526974903111022No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9560.21975293885075647No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9330.21446599576125086No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9170.21078812230768168No Hit
CATTTAAACCCTAATAAAACCAAACGATGGGGCTACTCCCTTAACTTCAT9110.20940891976259324No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA9010.20711024885411256No Hit
ACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCC8260.18987021704050716No Hit
GGGTAAAGGTGCAGATATTGGTTACACAGAAAGGCCTTATAAGTTGGCGA8190.18826114740457067No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA8180.1880312803137226No Hit
ATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT8100.18619234358693804No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7960.18297420431506503No Hit
CAGCCATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGT7800.17929633086149588No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7730.17768726122555936No Hit
TTATCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA7680.17653792577131902No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7660.17607819158962287No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7450.17125098268181338No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTTTCGCCAACTTATAAGGCC7300.1678029763190923No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7190.16527443831976352No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT7180.16504457122891544No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7160.1645848370472193No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAGGGTTTAATTCC7100.16320563450213085No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7060.16228616613873859No Hit
CCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC6890.15837842559432136No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGAATTAAAC6850.1574589572309291No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6790.15607975468584065No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA6770.1556200205041445No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6600.15171227995972728No Hit
ACAATGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6550.15056294450548693No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6530.1501032103237908No Hit
GGATAATAGGGTTTAATTCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6490.1491837419603985No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6360.14619546977937356No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6190.14228772923495636No Hit
GATTTGCCTCTGGCCAATGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA6110.14044879250817177No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6050.13906958996308336No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5900.13562158360036228No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5880.13516184941866613No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAT5800.13332291269188154No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5790.1330930456010335No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTG5710.1312541088742489No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5590.12849570378407205No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5570.1280359696023759No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5550.12757623542067978No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5490.12619703287559134No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5250.12068022269523761No Hit
AGACCATATTGTACAAAAAATCAAGCAATGTTTTCGAAAACTGCCTGTAA5190.11930102015014919No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5170.11884128596845304No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5000.11493354542403582No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4870.11194527324301089No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4840.11125567197046668No Hit
CTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGG4840.11125567197046668No Hit
CTCTTGGACTCTCAGCAATGTCAACAACCGACCTTGAGGCATACTTCAAA4830.1110258048796186No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA4810.11056607069792246No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGCCTCTGCCAAGTGTTTGCTG4770.10964660233453018No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4710.10826739978944175No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACAT4670.10734793142604945No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4620.10619859597180908No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4620.10619859597180908No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4540.10435965924502452No Hit
CTTATAAGGCCTTTCTGTGTAACCAATATCTGCACCTTTACCCCGTTGCC4490.10321032379078418No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4480.1029804566999361No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC4470.10275058960908803No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4420.10160125415484766No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA4420.10160125415484766No Hit
GATATTGGTTACACAGAAAGGCCTTATAAGTTGGCGAAAAAGTGAAAGCC4370.10045191870060731No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4360.10022205160975925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)