FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431253
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAGGGCATTAAACCTTAT21870.507126906943256No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGG18300.4243448741226148No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18180.42156228478410585No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG14890.3452729604199855No Hit
ATCCAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG14310.3318237786171922No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13180.305621062346233No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCA12970.3007515310038423No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA12520.29031682098443373No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12450.2886936438703035No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT10700.24811421601704803No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA10250.23767950599763943No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10240.2374476235527637No Hit
TTCCCCCACTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10080.23373750443475175No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT9720.2253897364192249No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9700.2249259715294734No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC9620.22307091197046747No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9120.21147678972668016No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9010.20892608283304698No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9000.2086942003881712No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCATTAAC8990.20846231794329545No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8940.20730290571891677No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8830.20475219882528356No Hit
GGATAATAAGGTTTAATGCCCTTATCCAAGGGCAAATATTTGGTAAGGTT8720.20220149193165035No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAGGGCATTAAAC8540.1980276079238869No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA8280.19199866435711752No Hit
CTTTAGGACATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA8190.1899117223532358No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7950.1843465436762179No Hit
CGCCTATGGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAA7920.18365089634159065No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7820.1813320718928332No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7810.18110018944795747No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT7750.179708894778703No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7710.1787813649992No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA7300.16927418475929443No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7270.1685785374246672No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA7270.1685785374246672No Hit
TTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT7180.16649159542078548No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT7050.16347712363740077No Hit
ATTGACAACAGTGCCAGCGGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6660.1544337082872467No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG6580.15257864872824073No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6490.15049170672435902No Hit
CATTTGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6480.15025982427948328No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT6390.14817288227560155No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC6320.14654970516147134No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6260.14515841049221687No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT6230.14446276315758963No Hit
CCCTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG6210.14399899826783813No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6140.14237582115370792No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA5830.1351874653625598No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5580.12939040424066614No Hit
CACAAAATGAAGCGCTGCGTGTAGTTTCTCTTTTATATAGAATGCCAGCC5550.12869475690603893No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAAAGGACAACAGAGTTGTCAGTC5530.12823099201628743No Hit
CCATCGGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATACTG5510.12776722712653593No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5430.12591216756752996No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5430.12591216756752996No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5310.12312957822902101No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5270.12220204844951804No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5190.12034698889051207No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5150.11941945911100908No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT5100.11826004688663035No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5040.11686875221737587No Hit
GGATAAGGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT4950.11478181021349416No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4950.11478181021349416No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA4950.11478181021349416No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC4910.11385428043399119No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACTGAGAGTCCAA4860.11269486820961246No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCACACAGTG4840.11223110331986097No Hit
GCTTTGGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG4770.11060792620573073No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4770.11060792620573073No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAAT4770.11060792620573073No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4720.10944851398135202No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4630.10736157197747029No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCGGTGTCGAGGAGATCTCGAA4560.10573839486334008No Hit
CTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGGTT4510.10457898263896136No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCCTTATCCAAGGGC4500.1043471001940856No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATATGTT4480.10388333530433412No Hit
GTTCAGGATAATAAGGTTTAATGCCCTTATCCAAGGGCAAATATTTGGTA4480.10388333530433412No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4450.10318768796970688No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4410.10226015819020388No Hit
TATTAACATTGACATAGCCGACTACTAATTCCCTGGATGCTGGGTCTTCC4390.10179639330045241No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4380.10156451085557665No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT4370.10133262841070091No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCCTTATCCAAGG4360.10110074596582518No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4340.10063698107607368No Hit
ATACCACATCATCCATATAACTGAAAGCCACACAGTGGGGGAAAGCCCTA4330.10040509863119791No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4330.10040509863119791No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)