FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656581
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC168592.567695379549515No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66501.0128224849637746No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC62740.955556130926725No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA43060.6558215970306787No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT38660.5888077784766845No Hit
ATACTATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA37560.572054323838186No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG35650.5429642344204294No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA33830.5152448822003682No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT30150.45919696122793685No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA29830.45432322896946453No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT27800.4234054899547809No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC27590.4202071031601585No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA27490.4186840618293859No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA26330.40101678239242383No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAAT25240.3844156318870025No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG24200.36857600204696755No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAATA23570.35898084166310024No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA23550.3586762333969457No Hit
ATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAATATGTT23280.3545640218038597No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAAT22950.3495379854123101No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT22780.3469488151499967No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC22640.34481655728691507No Hit
GGAACATATTGTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGTAA20720.3155741637360813No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC20130.306588219884523No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC20020.30491287442067316No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA19510.29714536363373295No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA19190.29227163137526063No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG17410.26516149568750846No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA17280.2631815419575041No Hit
GTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT16980.2586124179651863No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC16650.2535863815736368No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA16560.25221564437594146No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC16420.25008338651285983No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA15990.24353430879053764No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA15850.24140205092745604No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG15660.23850827239898809No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG15610.2377467517336018No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT15360.2339391484066703No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15040.22906541614819803No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC14940.22754237481742542No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTG14920.2272377665512709No Hit
TGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC14830.22586702935357555No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG14590.22221173015972134No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT14340.21840412683278987No Hit
GATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTAC14260.21718569376817182No Hit
CTTTACCACAGGAACATATTGTATTAAAACTCAAACAATGTTTTCGGAAA14110.2149011317720129No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC13680.20835205404969073No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATT13610.20728592511814994No Hit
GATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTAC13310.20271680112583218No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA13210.20119375979505955No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA13030.1984522853996689No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA13000.19799537300043712No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT12040.18337417622502022No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC11950.18200343902732488No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT11950.18200343902732488No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT11700.1781958357003934No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA11630.1771297067688526No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG11580.17636818610346627No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT11400.17362671170807562No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11350.17286519104268933No Hit
ATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT11330.1725605827765348No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG11070.16860067531652606No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT10640.1620515975942039No Hit
ATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTA10350.1576347777349634No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC10350.1576347777349634No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG10320.1571778653357316No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9910.15093339587956398No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT9640.14682118428647797No Hit
AACATATTGTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGTAAAT9490.1445366222903191No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG9460.14407970989108732No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATA9370.14270897269339197No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC9360.14255666856031474No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTT9290.14149053962877392No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA9290.14149053962877392No Hit
GCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAA9270.1411859313626194No Hit
CAGGAACATATTGTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGT9180.13981519416492405No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9130.13905367349953776No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA9030.13753063216876518No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG8990.13692141563645613No Hit
ATATTGTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGTAAATAGA8910.13570298257183805No Hit
TTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTA8530.1299154255149022No Hit
ATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACT8440.12854468831720686No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG8380.1276308635187433No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG8370.12747855938566605No Hit
CTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA8050.12260482712719374No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT8000.12184330646180744No Hit
AAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTAT7950.12108178579642115No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT7920.12062487339718937No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG7910.12047256926411211No Hit
CAGTTATATGGATGATATAGTATTGGGGGCCAAGTCTGTACAACATCTTG7690.11712187833641241No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7640.11636035767102612No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA7470.11377118740871271No Hit
GGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAA7450.1134665791425582No Hit
ACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGG7420.11300966674332642No Hit
CCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAA7370.11224814607794012No Hit
AATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAAT7300.1111820171463993No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG7240.11026819234793575No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA7220.10996358408178124No Hit
CCACAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGT7120.10844054275100862No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG7090.10798363035177684No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC7080.10783132621869959No Hit
GTTATATGGATGATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7070.10767902208562233No Hit
GTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCA6930.1055467642225407No Hit
GTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAG6880.1047852435571544No Hit
ACACGTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA6840.10417602702484537No Hit
CGTTTGGTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATT6820.10387141875869085No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC6770.10310989809330455No Hit
GCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTT6740.10265298569407277No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6650.10128224849637744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)