FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656581
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC153172.332842406344381No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65851.0029227163137526No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC65200.9930229476637308No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT36170.5508840493404469No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT34680.5281907335119354No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG33120.5044312887518829No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA32320.4922469581057022No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA31710.48295640598798933No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA30790.46894442574488143No Hit
ATACTATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA30080.45813083229639606No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT26910.4098504221109048No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA26340.401169086525501No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA25630.3903554930770156No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAATA25520.38868014761316577No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC25490.388223235213934No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA25450.387614018681625No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT23610.35959005819540923No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAAT21620.32928153571303465No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC21150.3221232414584035No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG21040.3204478959945536No Hit
ATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAATACAATATGTT20980.31953407119609006No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTAAT20650.3145080348045405No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA20620.31405112240530875No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC20470.3117665604091498No Hit
GGAACATATTGTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGTAA19330.29440388923834226No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA18830.28678868258447926No Hit
GTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT18270.2782596511321528No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT18270.2782596511321528No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC17370.26455227915519947No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC17170.26150619649365425No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA17140.26104928409442246No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA16370.2493218658474735No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC15970.24322970052438314No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT15730.23957440133052893No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT14370.21886103923202163No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14240.21688108550201723No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG14150.21551034830432195No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA14050.21398730697354934No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG13530.20606749205353186No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13450.2048490589889138No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG13380.20378293005737297No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATT13330.20302140939198668No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG13310.20271680112583218No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTG13280.20225988872660036No Hit
CTTTACCACAGGAACATATTGTATTAAAACTCAAACAATGTTTTCGGAAA13170.2005845432627505No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA13160.20043223912967326No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA12900.19647233166966452No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG12700.19342624900811933No Hit
GATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTAC12680.1931216407419648No Hit
TGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC12650.19266472834273302No Hit
GATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTAC12170.1853541299550246No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12020.1830695679588657No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA11880.1809373100957841No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA11210.1707329331796077No Hit
ATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT10930.16646841745344446No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT10920.16631611332036716No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG10900.16601150505421264No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA10880.16570689678805814No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT10420.15870090666650422No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC10390.15824399426727243No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT10380.15809169013419516No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT10080.1535225661418774No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATA9940.15139030827879574No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT9890.15062878761340945No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9690.14758270495186426No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG9650.14697348841955524No Hit
ATATTGTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGTAAATAGA9650.14697348841955524No Hit
GCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAA9560.1456027512218599No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9430.14362279749185553No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC9270.1411859313626194No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG9120.1389013693664605No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG8970.1366168073703016No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC8900.13555067843876079No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8590.13082925031336576No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT8340.12702164698643428No Hit
ATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTTTTA8200.12488938912335265No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG8190.12473708499027539No Hit
AACATATTGTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGTAAAT8120.12367095605873457No Hit
ATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACT8070.12290943539334825No Hit
GTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCA7890.1201679609979576No Hit
TTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTA7860.11971104859872582No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGTTTGAGTT7600.11575114113871708No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7390.11255275434409465No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7250.11042049648101301No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG7240.11026819234793575No Hit
CTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA7220.10996358408178124No Hit
GGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAA7200.10965897581562672No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC7180.1093543675494722No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA7180.1093543675494722No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG7170.10920206341639493No Hit
CAGTTATATGGATGATATAGTATTGGGGGCCAAGTCTGTACAACATCTTG7070.10767902208562233No Hit
CCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAA7040.10722210968639055No Hit
CAGGAACATATTGTATTAAAACTCAAACAATGTTTTCGGAAACTGCCTGT6970.10615598075484974No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6940.10569906835561796No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6860.1044806352909999No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC6840.10417602702484537No Hit
AAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTAT6780.10326220222638181No Hit
GTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTT6710.102196073294841No Hit
GTTATATGGATGATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAG6660.10143455262945471No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT6650.10128224849637744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)