FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786659
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40190.5108948095680593No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT31460.3999191517544451No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG24890.3164013886576013No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA18860.2397480992399502No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA15220.19347646184687395No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTTTCA14820.18839166652895345No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG14390.18292551156218895No Hit
CTCTTACAGTTAATGAAAAAAGAAGATTAAAATTAATTATGCCTGCTAGG14210.18063735366912476No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13870.17631527764889232No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA13110.16665416654484344No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA12730.161823610992819No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12220.15534049696247038No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT11980.15228961977171812No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTTTCA11330.14402682738009734No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT11270.14326410808240927No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11180.14212002913587718No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT11040.140340350774605No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10790.1371623537009047No Hit
GTTTTCCACTCAGGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGT10710.13614539463732062No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10690.13589115487142459No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA10620.13500131569078852No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC10440.1327131577977243No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10240.13017076013876408No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA9490.12063676891766319No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9480.12050964903471517No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9400.11949268997113106No Hit
GTTACTATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG9320.11847573090754698No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9080.11542485371679469No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8830.1122468566430944No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8680.11034005839887422No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8530.10843326015465406No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGAGCAGCA8120.10322134495378557No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTTTCATTAAC7920.10067894729482534No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA7920.10067894729482534No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7870.10004334788008527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)