FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786659
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40080.5094964908556312No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT30270.3847918856836317No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG25210.3204692249119377No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA19800.2516973682370633No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA16990.21597668112867202No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTTTCA15970.2030104530679748No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA14700.18686622793357732No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG14420.18330687121103298No Hit
CTCTTACAGTTAATGAAAAAAGAAGATTAAAATTAATTATGCCTGCTAGG14200.18051023378617673No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13340.16957792385264772No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12450.15826425427027466No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA12170.15470489754773034No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT12080.15356081860119822No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT11720.1489845028150698No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11590.14733194433674565No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11520.14644210515610956No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC11170.14199290925292915No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11120.1413573098381891No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTTTCA11090.14097595018934506No Hit
GTTTTCCACTCAGGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGT11050.14046747065755302No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA10160.12915380107517996No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10060.12788260224569986No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10040.12762836247980383No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9840.12508596482084358No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA9810.12470460517199956No Hit
GTTACTATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG9610.12216220751303931No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9120.11593333324858675No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8850.11250109640899043No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8800.11186549699425037No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8660.1100858186329782No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA8630.10970445898413417No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTTTCATTAAC8490.10792478062286201No Hit
GTCTATTTACAGGCAGTTTCCGAAAACAATGCTTGATTTTTAATACAATA8440.10728918120812195No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8280.10525526308095376No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8270.10512814319800574No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC8070.1025857455390455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)