FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408049
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG35080.8597006732034633No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT30820.7553014466399869No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA26630.652617700325206No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG21250.5207707897825996No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19730.48352036152520894No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT18220.44651500187477483No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT17310.4242137586417317No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT13790.3379496089930376No Hit
CTATCAATAATGTCCTCCTGTAAGTGAATGTTAGGAAAGGAGGGAGTTTG12890.31589343436695105No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12290.30118931794955994No Hit
CTATTAATAATGTCCTCCTGTAAGTGAATGTTAGGAAAGGAGGGAGTTTG11960.2931020539199949No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA11160.2734965653634735No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA10590.259527654766952No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10590.259527654766952No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA9990.24482353834956094No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9310.22815887307651778No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8920.21860119740521355No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8490.20806324730608333No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8260.20242666934608342No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7990.19580981695825747No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7930.19433940531651836No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7780.1906633762121706No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7750.18992817039130105No Hit
GTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG7670.1879676215356489No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC7500.1838014552173881No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCACTTACAGG7490.1835563866104316No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7470.18306624939651855No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7130.17473391675999697No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7110.17424377954608392No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6950.17032268183477964No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATACCCACATTGTGTAAAA6930.1698325446208666No Hit
CTATATAAGAGAGAAACTACACGCAGCGCGTCATTTTGTGGGTCACCATA6880.16860720158608403No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT6740.16517624108869278No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA6540.16027486894956242No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6460.1583143200939103No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6380.15635377123825817No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6240.15292281074086692No Hit
TACCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5930.14532568392521486No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT5860.14361020367651922No Hit
CATTTGAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT5850.14336513506956272No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGG5780.1416496548208671No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5760.14115951760695408No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5610.1374834885026063No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5600.13723841989564978No Hit
CATATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTT5350.13111170472173686No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA5320.1303764989008673No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG5250.12866101865217167No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5210.1276807442243456No Hit
CATATAAAGGCATCAAGGCAGGATACCCACATTGTGTAAAAGGGGCAGCA5130.12572019536869347No Hit
AGTTAAGGGAGTAGCCCCAACGCTTGGTTTTATTAGGGTTCAAATGTATA5110.12523005815478044No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5110.12523005815478044No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG5090.12473992094086739No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG5030.12326950929912829No Hit
ATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAG5020.12302444069217178No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT5000.12253430347825875No Hit
GAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACCT4930.12081882322956311No Hit
GTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGAT4930.12081882322956311No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC4870.119348411587824No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGA4830.11836813715999793No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGT4790.11738786273217189No Hit
CCAATTGGACCCTGCGTTCGGAGCCAACTCAAACAATCCAGATTGGGACT4740.11616251969738928No Hit
ATATAAGAGAGAAACTACACGCAGCGCGTCATTTTGTGGGTCACCATATT4730.11591745109043278No Hit
GCGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA4720.11567238248347625No Hit
ATATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG4640.11371183362782411No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAAT4530.11101607895130242No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTC4520.11077101034434589No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4510.11052594173738939No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4490.11003580452347635No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4490.11003580452347635No Hit
CTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTA4480.10979073591651982No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT4460.10930059870260678No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4450.10905553009565028No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4340.1063597754191286No Hit
CTTTTACACAATGTGGGTATCCTGCCTTGATGCCTTTATATGCATGTATA4300.10537950099130253No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCATGGTGGTCTCCATGC4280.10488936377738949No Hit
GGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAATTAATTT4280.10488936377738949No Hit
GATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT4240.10390908934956342No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4220.10341895213565037No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4210.10317388352869385No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAA4200.10292881492173735No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4190.10268374631478082No Hit
AATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCCTTGG4180.1024386777078243No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATATGTT4170.10219360910086778No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4160.10194854049391128No Hit
ACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA4150.10170347188695475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)