FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435879
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23480.5386816065926553No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG16500.37854542200931907No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT14920.34229683008357825No Hit
ATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTAT14300.32807269907474323No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11770.270029067699981No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11270.2585579943057592No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10550.24203964861807978No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10540.24181022715019534No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9990.2291920464165514No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT9690.22230940238001834No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9640.22116229504059612No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG9250.21221485779310315No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8500.19500824770177044No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTATTACCAATTTTCTT8190.18789618219735293No Hit
GTTCTATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAAC8070.1851431245827397No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7770.17826048054620663No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7730.17734279467466887No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7560.17344262972063346No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7370.1690836218308292No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7320.16793651449140704No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTAAGGTT6950.1594479201796829No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6830.15669486256506968No Hit
GAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6630.15210643320738096No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC6600.15141816880372763No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6400.14682973944603891No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6340.14545321063873232No Hit
CCTAAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG6070.13925883100585254No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG6010.13788230219854591No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5940.13627635192335488No Hit
CATGTATACAATCTAAGCAAGCTTTCACTTTCTCGCCAACTTACAAGGCC5870.13467040164816382No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5870.13467040164816382No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5690.13054081522624397No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT5680.13031139375835954No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5600.12847602201528407No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5450.12503469999701752No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT5440.12480527852913308No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5360.1229699067860576No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5330.1222816423824043No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5300.121593377978751No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCA5280.12113453504298212No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGTAAATATTTGGTA5150.11815205596048443No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4980.11425189100644904No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4970.1140224695385646No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4970.1140224695385646No Hit
GGCCTCTACCGTCCCCTTCTTCAGCTGCCGTTCCGGCCGACCACGGGGCG4960.11379304807068018No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAATA4940.1133342051349113No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4890.11218709779548912No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT4710.10805751137356928No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4640.10645156109837822No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA4620.10599271816260936No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4620.10599271816260936No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGT4600.10553387522684048No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTAATGAAA4510.10346908201588055No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC4480.10278081761222724No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTCATTACAATATGTT4480.10278081761222724No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4390.10071602440126734No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4390.10071602440126734No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCGCTAT4390.10071602440126734No Hit
ATATTTACCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAG4360.10002775999761401No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4360.10002775999761401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)