FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498293
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG18190.3650462679588114No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15100.3030345599877983No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14960.3002249680408916No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATCAAACCGTAT14460.29019071108765326No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14310.28718043400168175No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT12720.25527149689038375No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA10880.2183454313024666No Hit
GGTTAGGATAGAACCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10470.21011734060081116No Hit
CTCCAAAAGACACTAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10390.208511859488293No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA10040.20148787962102616No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATCAAAC10030.20128719448196142No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10000.20068513906476715No Hit
CTATTAATAATGTCCTCCCGCAAATGAATGTGAGGAAAGGAGGGAGTTTG9820.1970728065616013No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTCACAGTTAATGAAAAA9740.19546732544908318No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9610.19285841864124117No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9580.1922563632240469No Hit
AGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACTAAATACTCAAGAA9390.18844334558181633No Hit
CATGTATACAATCTAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9330.18723923474742774No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC9080.18222210627080854No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8920.17901114404577226No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8530.17118442362224637No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8010.16074879639087844No Hit
GTTTTAGTACAATATGGTCTTGTGGTAAAGTACCCCAACTTCCAATTACA7900.15854125986116602No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7810.15673509360958313No Hit
CTCTCACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCAAGG7740.15533029763612974No Hit
GTACAATATGGTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC7600.15252070568922302No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC7570.15191865027202872No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7550.15151727999389916No Hit
AGTCTAGACTCTGAGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7480.1501124840204458No Hit
GTCCCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGAT7440.14930974346418674No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7430.14910905832512197No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7340.14730289207353905No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7320.14690152179540952No Hit
GTACTAAAACTCAAGCAATGTTTTCGTAAACTGCCTGTAAATAGACCTAT7180.14409192984850278No Hit
GGATAATACGGTTTGATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT7130.14308850415317895No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6970.13987754192814267No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6940.13927548651094837No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6840.1372686351203007No Hit
CTGTGAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCCGC6800.13646589456404165No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCGGG6770.13586383914684735No Hit
GGATAGAACCTTGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6760.13566315400778256No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTG6740.13526178372965303No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6700.13445904317339397No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6680.13405767289526443No Hit
CTGCTAGACCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAG6640.13325493233900537No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC6640.13325493233900537No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6540.1312480809483577No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6520.13084671067022816No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6510.1306460255311634No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6420.1288398592795805No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6370.12783643358425667No Hit
ATATGGTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6330.12703369302799758No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6290.12623095247173852No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6270.12582958219360896No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6130.12301999024670225No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6130.12301999024670225No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6050.1214145091341841No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6010.12061176857792502No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACTAAATACTCAAGAA5980.12000971316073072No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5970.11980902802166597No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTA5960.11960834288260119No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5790.11619669551850016No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5750.11539395496224109No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5740.11519326982317632No Hit
ACCCATCTCGGGGCCGTTTGGGGCTCTACCGTCCCCTTCTTCAGCTGCCG5730.11499258468411157No Hit
GTTCAGGATAATACGGTTTGATGCCTTTGTCCAAGGGCAAATATTTGGTA5670.11378847384972296No Hit
GTCTATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTAGTACAATA5670.11378847384972296No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5670.11378847384972296No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5670.11378847384972296No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5650.11338710357159341No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5640.11318641843252866No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5600.11238367787626959No Hit
GGACAAATCTTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAG5560.11158093732001051No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5460.10957408592936284No Hit
ATTTACAGGCAGTTTACGAAAACATTGCTTGAGTTTTAGTACAATATGGT5400.10836997509497426No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTACGAAAAC5260.10556038314806751No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5230.1049583277308732No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGTAAACTGCCTGTAAATAGA5180.10395490203554936No Hit
GTCTACGTCCCCTCGGCGCTGAATCCCGCGGACGACCCATCTCGGGGCCG5160.10355353175741983No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5120.10275079120116078No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5100.10234942092303123No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5050.1013459952277074No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5040.10114531008864262No Hit
GGATGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC5030.10094462494957786No Hit
TCCTTTGTCTACGTCCCCTCGGCGCTGAATCCCGCGGACGACCCATCTCG5000.10034256953238357No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4990.1001418843933188No Hit
CCTCTTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4990.1001418843933188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)