FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460365
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17570.3816536878346529No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC13430.2917250442583602No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12220.265441551812149No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10870.23611699412422754No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT10800.2345964615033723No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10270.22308385737404018No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10130.22004279213232977No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGAAAGCCACATTGTGTAAAA9890.21482953743225483No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9680.21026793956968928No Hit
CTCCTAGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA9570.20787853116548827No Hit
GTGTAAAAGGGGCAGCAAAGCCTAAAAGACCCACAATTCGTTGACATACT9560.20766131221965178No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT8910.19354208074028217No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8570.18615663658184267No Hit
CTGTAAGAGGTCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8280.17985728715258545No Hit
TTATTAATAGATGTCAACAATATGTGGGACCTCTTACAGTTAATGAAAAA7980.17334071877749177No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7930.1722546240483095No Hit
CTCCAAAAGACACCAAATACTCCAGAACAGTTTCTCTTCCAAAAGTAAGA7910.17182018615663658No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7820.16986521564410847No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7730.16791024513158037No Hit
GTCTAGGAGATCTCGAATAGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGA7150.15531154627306595No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6970.15140160524800972No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6950.15096716735633683No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6920.15031551051882744No Hit
GGGTAAAGGTGCAGATATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA6870.14922941578964516No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6780.14727444527711706No Hit
GCCTAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTAT6760.14684000738544417No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6700.14553669371042544No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6620.14379894214373376No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6570.14271284741455148No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6460.1403234390103505No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC6390.1388029063894953No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6200.13467574641860264No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT6190.13445852747276618No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6160.1338068706352568No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5880.12772474015183605No Hit
TCCTCAGGCCATGCAGTGGAATTCCACCACATTCCATCAAGCTCTGCTAG5660.12294592334343403No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5600.12164260966841527No Hit
TGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGG5570.12099095283090591No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5560.12077373388506947No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5550.12055651493923299No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5370.1166465739141768No Hit
CTTTTACACAATGTGGCTTTCCTGCCTTGATGCCTTTATATGCATGTATA5340.11599491707666743No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5130.11143331921410185No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5100.1107816623765925No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTTGTACAATA5100.1107816623765925No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4990.10839225397239147No Hit
TCCCGATACAGAGCAGAGGCGGTGTCTAGGAGATCTCGAATAGAAGGAAA4940.10730615924320919No Hit
CATATAAAGGCATCAAGGCAGGAAAGCCACATTGTGTAAAAGGGGCAGCA4930.10708894029737273No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4910.10665450240569983No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATCTTT4900.10643728345986338No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4880.10600284556819045No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4810.10448231294733527No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4790.10404787505566236No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4730.10274456138064361No Hit
TGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCG4730.10274456138064361No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4710.10231012348897071No Hit
ATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTGACTACT4630.10057237192227905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)