FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422797
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG20800.4919618634947741No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT15610.36920791774776074No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGATCATATTATCCAAAAA15310.3621123139473553No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA11260.2663216626418825No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC11210.2651390620084816No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG11110.2627738607416798No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11030.2608816997282384No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10860.2568608575746753No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC10180.24077748896042306No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10060.23793924744026093No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9940.23510100592009875No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9850.23297232477997717No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC9820.23226276439993662No Hit
CCTCCCGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9760.23084364363985554No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA8870.2097933523653195No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8830.2088472718585988No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8570.20269774856491413No Hit
CTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTCCTAAT8410.19891342653803123No Hit
GGATCATATTATCCAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA8260.19536562463782856No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG8000.18921610134414388No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT7830.18519525919058083No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCG7790.1842491786838601No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACAT7700.1821204975437385No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7620.18022833653029705No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA7610.17999181640361686No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC7580.17928225602357634No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7560.17880921577021597No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7540.1783361755168556No Hit
CATGTATACAATCTAAGCAGGCCTTCACTTTCTCGCCAACTTACAAGGCC7490.1771535748834547No Hit
ATGTGATTGGAAGTTGGGGTACTTTACCACAGGATCATATTATCCAAAAA7300.1726596924765313No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7190.1700579710830493No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA7190.1700579710830493No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7090.1676927698162475No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6970.16485452829608535No Hit
CAATCACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTTGTTTTA6480.15326504208875655No Hit
CTTTACCACAGGATCATATTATCCAAAAACTCAAGCAATGTTTTCGAAAA6380.15089984082195473No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6310.1492441999351935No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG6210.14687899866839169No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG6180.14616943828835116No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA6110.1445137974015899No Hit
ATGCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA6090.14404075714822953No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA6080.14380423702154935No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6070.14356771689486916No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6050.1430946766415088No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6050.1430946766415088No Hit
CTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6010.14214859613478809No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5980.14143903575474756No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5940.14049295524802682No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5700.13481647220770251No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5660.1338703917009818No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCGCACTCAGGCA5630.13316083132094125No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCG5580.13197823068754036No Hit
GCAGACGAAGAAGGGGACGGTAGAGGTTCAAACGGCCCCGAGACGGGTCG5520.13055910992745928No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5450.12890346904069802No Hit
GTGCTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5390.12748434828061692No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5330.12606522752053587No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5250.12417306650709442No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCGTCTGCGAC5220.12346350612705388No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5170.12228090549365297No Hit
ATTGACAACAGTGCCAGCTGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5160.1220443853669728No Hit
TTCTTCTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTC5000.11826006334008993No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5000.11826006334008993No Hit
CTCCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAG4930.11660442245332867No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4930.11660442245332867No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4900.11589486207328813No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACAC4900.11589486207328813No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4870.11518530169324759No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4870.11518530169324759No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT4820.11400270105984668No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4810.11376618093316651No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4800.11352966080648633No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4790.11329314067980614No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA4780.11305662055312597No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4700.11116445953968453No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTTGTTTTA4670.11045489915964399No Hit
GATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGT4670.11045489915964399No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4660.11021837903296382No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4660.11021837903296382No Hit
CTATTGTGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT4590.10856273814620254No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4570.10808969789284219No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4540.10738013751280165No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4470.10572449662604039No Hit
ATCCTGCTCTGATGCCTTTATATGCATGTATACAATCTAAGCAGGCCTTC4460.10548797649936022No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4430.10477841611931968No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC4430.10477841611931968No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGACA4420.1045418959926395No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4420.1045418959926395No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4420.1045418959926395No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTATTTACAGGCAATTTTCG4410.10430537586595932No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4350.10288625510587823No Hit
GTGTTGGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4350.10288625510587823No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCTGGTC4330.10241321485251788No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4320.1021766947258377No Hit
ATCTAAGCAGGCCTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4270.10099409409243679No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)