FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422797
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG23140.5473075731379362No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13930.3294725364654905No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGATCATATTATCCAAAAA13640.3226134527917653No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12650.29919796025042755No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12260.2899736753099005No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA11900.281458950749414No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC10840.25638781732131494No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10800.25544173681459426No Hit
CCTCCCGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10270.2429061701005447No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC10170.2405409688337429No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG10130.2395948883270222No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9890.23391840528669788No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT9420.22280195933272942No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA9240.21854459705248616No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8940.21144899325208077No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG8860.20955683223863936No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT8820.20861075173191865No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8630.2041168693249952No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8560.20246122843823397No Hit
GGATCATATTATCCAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA8360.19773082590463037No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8070.19087174223090514No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7950.18803350071074298No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCG7860.18590481957062138No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACAT7790.1842491786838601No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7710.18235701767041868No Hit
CTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTCCTAAT7540.1783361755168556No Hit
CATGTATACAATCTAAGCAGGCCTTCACTTTCTCGCCAACTTACAAGGCC7170.16958493082968895No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7120.16840233019628806No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCA7070.16721972956288714No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC6970.16485452829608535No Hit
GAGTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCA6940.1641449679160448No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6790.16059716601584212No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA6770.16012412576248175No Hit
ATGTGATTGGAAGTTGGGGTACTTTACCACAGGATCATATTATCCAAAAA6700.1584684848757205No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6650.1572858842423196No Hit
GCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTC6630.15681284398895926No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCG6200.1466424785417115No Hit
CTTTACCACAGGATCATATTATCCAAAAACTCAAGCAATGTTTTCGAAAA6090.14404075714822953No Hit
ATGCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA6080.14380423702154935No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG5980.14143903575474756No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5980.14143903575474756No Hit
CAATCACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTTGTTTTA5960.1409659955013872No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACCGCACTCAGGCA5840.138127753981225No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5820.13765471372786467No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5790.13694515334782412No Hit
GTGCTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5740.13576255271442322No Hit
CTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5660.1338703917009818No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCGTCTGCGAC5630.13316083132094125No Hit
CTCCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAG5570.13174171056086018No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA5550.1312686703074998No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5550.1312686703074998No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5520.13055910992745928No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG5480.12961302942073857No Hit
TTCTTCTACTGTACCTGTCTTTAATCCGGAGTGGCAAACTCCCTCCTTTC5470.12937650929405836No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5430.12843042878733765No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5430.12843042878733765No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT5400.12772086840729713No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5370.12701130802725658No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5340.12630174764721602No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5270.12464610676045478No Hit
GATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGT5160.1220443853669728No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATA5150.12180786524029262No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5110.12086178473357191No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5070.11991570422685117No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4970.11755050296004937No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4950.11707746270668902No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4920.11636790232664848No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG4830.11423922118652687No Hit
ATTGACAACAGTGCCAGCTGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4810.11376618093316651No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4810.11376618093316651No Hit
CTCTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4800.11352966080648633No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4800.11352966080648633No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT4740.11211054004640524No Hit
GTATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA4730.11187401991972507No Hit
GAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCC4730.11187401991972507No Hit
GCTATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT4700.11116445953968453No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTATTTACAGGCAATTTTCG4610.10903577839956292No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4580.10832621801952237No Hit
GTGTTGGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4550.10761665763948183No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA4510.10667057713276112No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4470.10572449662604039No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4450.10525145637268003No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4440.10501493624599985No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4430.10477841611931968No Hit
GCAGACGAAGAAGGGGACGGTAGAGGTTCAAACGGCCCCGAGACGGGTCG4420.1045418959926395No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGACA4380.10359581548591877No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4350.10288625510587823No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4350.10288625510587823No Hit
CTATTGTGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT4340.10264973497919805No Hit
CCCTTGGACTCATAAGGTGGGGAACTTTACTGGGCTTTATTCTTCTACTG4320.1021766947258377No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGTGGAG4250.10052105383907643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)