FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443547
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT25860.5830272778307597No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG25420.5731072468081173No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT24020.5415435117360731No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG19830.44707776177045505No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT15610.35193564605329314No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA15360.3462992647904281No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15120.3408883387780776No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA14080.31744099272455906No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13120.2957972886751573No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC13050.2942191019215551No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12470.2811426973917082No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10830.2441680363073135No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10710.2414625733011383No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTT9980.22500434001357242No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGC9940.224102519011514No Hit
CTCCGAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8320.18757876842814855No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGCCCCACAATTCTTTGACATACT8120.1830696634178565No Hit
TGATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGG8110.1828442081673419No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGCTGGGGTACTTTACCGCAG8030.1810405661632251No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7970.1796878346601375No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTA7950.1792369241591083No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA7840.17675691640344765No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7810.17608055065190387No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGGAGGGAGTTTG7790.17562964015087465No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7640.17224781139315562No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7580.17089507989006802No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7380.166385974879776No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCTACCAATCGGCAGT7150.16120050411794015No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA7120.16052413836639634No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG7120.16052413836639634No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7040.15872049636227953No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6870.1548877571035313No Hit
GATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGC6830.1539859361014729No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6820.1537604808509583No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6710.15128047309529769No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG6710.15128047309529769No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6640.14970228634169547No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6480.14609500233346184No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6280.1415858973231698No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6250.140909531571626No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6010.13549860555927556No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5990.13504769505824638No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA5960.13437132930670256No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC5940.13392041880567335No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5930.13369496355515875No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT5930.13369496355515875No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCGTACCTGTC5910.13324405305412954No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5850.13189132155104194No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5790.13053859004795434No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG5760.12986222429641053No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA5740.12941131379538132No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTTGTACAATA5720.12896040329435213No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5670.12783312704177913No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5650.1273822165407499No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5610.1264803955386915No Hit
ATATGTTCCTGCGGTAAAGTACCCCAGCTTCCAATTACATATCCCATGAA5590.1260294850376623No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTA5590.1260294850376623No Hit
ATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGG5510.12422584303354547No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5430.12242220102942868No Hit
ACCCATCTCGGGGCCGCTTGGGACTCTACCGTCCCCTTCTTCTGCTGCCG5400.12174583527788486No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC5370.12106946952634107No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCG5280.11904037227170965No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5230.11791309601913663No Hit
GCATCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT5130.11565854351399062No Hit
GCGGTAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCAAGAAC5050.11385490150987382No Hit
ATGTAATTGGAAGCTGGGGTACTTTACCGCAGGAACATATTGTACAAAAA4930.1111494385036986No Hit
GTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCT4850.10934579649958177No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4850.10934579649958177No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC4780.10776760974597957No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4730.10664033349340656No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG4680.10551305724083357No Hit
CCCTTAGACAAAGGCATTAAACCGTATTATCCTGAACATGCAGTTAATCA4670.10528760199031895No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4650.10483669148928976No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4600.10370941523671674No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC4600.10370941523671674No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4580.10325850473568753No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA4570.10303304948517293No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4560.10280759423465834No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT4560.10280759423465834No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTTCACCAAGCTCTGCTAG4490.10122940748105612No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA4460.10055304172951231No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)