FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences476817
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT15950.3345098853438531No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG15650.328218163362464No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG14290.29969569038016686No Hit
TTATTAATAGATGTCAACAATTTGTGGGCCCTCTTACAGTTAATGAAAAA14260.2990665181820279No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13490.2829177650964626No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA13270.2783038356434439No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12600.26425232321834163No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAA12170.2552341883783506No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11860.24873274233091522No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAAT11290.23677847056627596No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10010.2099337901123492No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10000.20972406604630286No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9830.20615875692351573No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT9700.2034323440649138No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA9670.2028031718667749No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCCTCCTGT9580.20091565527235816No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA9580.20091565527235816No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC9390.1969308980174784No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9320.1954628295551543No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9280.19462393329096908No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8650.181411317130052No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAAGCC8040.16861814910122752No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7960.16694035657285708No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7640.1602291864593754No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7390.15498608480821782No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7330.15372774041194No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCTTTGTAAGTTGGCGA6940.14554850183613421No Hit
GCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTA6850.14366098524171747No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6700.14051512425102292No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA6610.1386276076566062No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6530.13694981512823579No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT6370.13359423007149493No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6280.1317067134770782No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA6080.12751223215615215No Hit
GATAACCACATTGTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACAATT6020.12625388775987434No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5940.12457609523150391No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5910.12394692303336499No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT5900.1237371989673187No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5830.12226913050499458No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5770.12101078610871677No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAACAG5600.11744547698592962No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5490.11513851225942028No Hit
GAATATGGTGACCCACAAAATGAAGCGCTACGTGTAGTTTCTCTTTTATA5430.11388016786314245No Hit
CATATAAAGGCATCAAGGCAGGATAACCACATTGTGTAAAAGGAGCAGCA5410.11346071973104986No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5370.11262182346686465No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5360.11241209940081834No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5290.11094403093849423No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5240.10989541060826272No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT5230.1096856865422164No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5190.1088467902780312No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA5190.1088467902780312No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5170.10842734214593858No Hit
CTTTACCACAGGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAA5030.10549120522129035No Hit
GTGTAGTTTCTCTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA4950.10381341269291991No Hit
ATATAAAAGAGAAACTACACGTAGCGCTTCATTTTGTGGGTCACCATATT4820.10108699983431797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)