FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689726
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG40730.5905243531489316No Hit
ATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTT40060.5808103507769752No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT32860.47642107155595126No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27400.397259201480008No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG27210.39450448438945324No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT23900.34651441296978797No Hit
GATTACAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT21290.3086732992521668No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA20860.3024389395209112No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG19860.28794042851799123No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG19810.2872155029678452No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT19410.2814160985666772No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTTGGTCTTTTGG19260.2792413219162392No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT18710.2712671408646332No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT16310.23647071445762521No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA16060.2328460867068952No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC15750.22835154829599003No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14310.20747369245178524No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA14290.2071837222317268No Hit
AGCGTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA13870.20109434761050043No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCAACAATTCTTTGACATACT13480.1954399283193616No Hit
GTATGTAAATAATGCATAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13420.1945700176591864No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA13310.1929751814488652No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA13240.1919602856786608No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13130.1903654494683396No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC13100.189930494138252No Hit
ATTACAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATATT12830.18601589616746358No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTACAATTAATTATGCCTGCTAGG12820.1858709110574344No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG12780.18529097061731759No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG12280.1780417151158576No Hit
GATTAAGATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT12180.1765918640155656No Hit
ACTATGCATTATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAG11740.1702125191742808No Hit
AAGTAAGACAGGAAATGTGAAACCACAATAGTTGTCTGATTTTTAGGCCC11460.16615293609346318No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11260.1632532338928792No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC11050.160208546582266No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC11040.1600635614722368No Hit
GATTAAACTTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT10800.156583918831536No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC10600.153684216630952No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA10600.153684216630952No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10380.1504945442103096No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTTGGTCTTTTGG10300.149334663330076No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA10170.1474498568996964No Hit
TTATCAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA10150.147159886679638No Hit
CTCTCACAGTTAATGAAAAGAGGAGATTAAATTTAATTATGCCTGCTAGG10050.145710035579346No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC10040.1455650504693168No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT9980.14469513980914162No Hit
ATTGTAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGA9910.1436802440389372No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9840.1426653482687328No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG9780.1417954376085576No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9460.1371559140876232No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9420.1365759736475064No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA9360.1357060629873312No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG9220.1336762714469224No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC9140.1325163905666888No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA9020.1307765692463384No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC8800.127586896825696No Hit
GGGGTCGTTGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTAG8710.1262820308354332No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC8660.1255571052852872No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT8580.12439722440505359No Hit
CTCTCACAGTTAATGAAAAGAGGAGATTAAAATTAATTATGCCTGCTAGG8510.1233823286348492No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT8330.1207725966543236No Hit
ACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTATATTTTCC8270.11990268599414841No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA8240.1194677306640608No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8230.1193227455540316No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT8180.1185978200038856No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA8070.1170029837935644No Hit
CATTTGAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7970.1155531326932724No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT7970.1155531326932724No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA7950.115263162473214No Hit
GTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGG7950.115263162473214No Hit
TTATCAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGA7800.113088385822776No Hit
GGAAAGAATCCCAGAGGATTGGGCACAGAAAGATTCGTCCCCATGCCTTG7690.11149354961245479No Hit
TCCGAAAACATTGCTTGAGTTTTAATACAATATGTTCCTGTGGTAAAGTA7670.1112035793923964No Hit
GAATATGGTGACCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTTTTATA7560.10960874318207521No Hit
GGTCGATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT7430.10772393675169559No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7420.10757895164166639No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7370.1068540260915204No Hit
AGGCCAATCAGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACCCCACCA7370.1068540260915204No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC7320.1061291005413744No Hit
AGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTG7280.1055491601012576No Hit
CGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTA7250.10511420477116999No Hit
CTCCAAAAGACACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTAAGA7190.1042442941109948No Hit
ATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAG7070.1025044727906444No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7060.1023594876806152No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7020.1017795472404984No Hit
AGGTCACCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATC7000.10148957702044001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)