FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689726
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG48430.7021628878714157No Hit
ATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTT36390.5276008153962588No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT29300.424806372385556No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG27750.40233368033103No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27300.39580935037971604No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT24170.35042901094057644No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT20190.2927249371489548No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG19860.28794042851799123No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG19810.2872155029678452No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT19420.2815610836767064No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT18430.2672075577838156No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA18320.2656127215734944No Hit
GATTACAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT17610.2553187787614212No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTTGGTCTTTTGG17530.2541588978811876No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC17400.252274091450808No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA16960.24589474660952318No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCAACAATTCTTTGACATACT15090.2187825310340628No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA14750.21385303729306998No Hit
GTATGTAAATAATGCATAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14210.2060238413514932No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC13780.1997894816202376No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13230.19181530056863158No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12700.184131089737084No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG12240.1774617746757408No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG11800.171082429834456No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11780.1707924596143976No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11490.1665878914235508No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTACAATTAATTATGCCTGCTAGG11420.1655729956533464No Hit
AGCGTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA11250.16310824878285002No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC11120.1612234423524704No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA10990.1593386359220908No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC10830.1570188741616236No Hit
ATTACAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATATT10620.1539741868510104No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA10610.1538292017409812No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG10530.1526693208607476No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10430.1512194697604556No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC10250.14860973777993No Hit
GATTAAGATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT9740.14121549716844078No Hit
CTCTCACAGTTAATGAAAAGAGGAGATTAAATTTAATTATGCCTGCTAGG9380.1359960332073896No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA9370.1358510480973604No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTTGGTCTTTTGG9320.1351261225472144No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9250.13411122677700998No Hit
TTATCAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA9150.132661375676718No Hit
GATTAAACTTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT9100.131936450126572No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA8900.129036747925988No Hit
AAGTAAGACAGGAAATGTGAAACCACAATAGTTGTCTGATTTTTAGGCCC8880.1287467777059296No Hit
ACTATGCATTATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAG8810.12773188193572518No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT8780.1272969266056376No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8650.12541212017525802No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA8530.12367229885490759No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG8240.1194677306640608No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT8140.11801787956376879No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC8130.1178728944537396No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8100.11743793912365201No Hit
ATTGTAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGA7930.11497319225315561No Hit
CTCTCACAGTTAATGAAAAGAGGAGATTAAAATTAATTATGCCTGCTAGG7880.11424826670300961No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC7830.11352334115286361No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7760.1125084453826592No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA7750.11236346027263No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC7720.1119285049425424No Hit
TTATCAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGA7660.11105859428236721No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA7590.1100436985121628No Hit
GTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGG7560.10960874318207521No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT7390.10714399631157881No Hit
GGTCGATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT7330.1062740856514036No Hit
GAATATGGTGACCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTTTTATA7320.1061291005413744No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA7300.105839130321316No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA7140.1035193685608488No Hit
CTCCAAAAGACACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTAAGA7130.1033743834508196No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA7100.10293942812073201No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG7100.10293942812073201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)