FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299057
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15420.5156207679472475No Hit
CATCTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTAAACTTCAT14340.47950725112603954No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG14220.4754946381459053No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA11490.3842076928478517No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG11380.3805294642827287No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10850.3628070902871359No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTAAACTTCAT10070.3367251059162634No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA9240.3089711994703351No Hit
GGTTGCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG8950.2992740514350107No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCAGACCTTTAACCT8460.28288921509946263No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8410.2812172930244067No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8120.27152014498908233No Hit
CTATCGGCCATCGCCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATC8030.2685106852539817No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8010.2678419164239593No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTCTGACATACT7950.26583560993389216No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7900.26416368785883626No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7800.26081984370872446No Hit
GGCCAATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG7620.2548009242385231No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7500.25078831125838885No Hit
GTTCAGATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA7500.25078831125838885No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCACCAACTTACAAGGCC7490.2504539268433777No Hit
CTCCAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAA7230.24175993205308688No Hit
CCCTAAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA6930.23172839960275132No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5950.19895872693165517No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCCGCTAGGAGTTCCGCA5930.1982899581016328No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA5820.19461172953650976No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA5710.19093350097138673No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5510.18424581267116302No Hit
GAGCAAAACTTATCGGAACCGACAACTCTGTTGTCCTCTCTCGGAAATAC5490.18357704384114065No Hit
GTCTTACATAAGAGGACTCTTGGACTCGCAGCAATGTCAACGACCGACCT5360.17923004644599524No Hit
GAGACACTGTGCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACT5340.17856127761597287No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5240.17521743346586102No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5180.1732111269757939No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5110.17087043607071561No Hit
TTCCATGGCTGCTAGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4990.1668578230905814No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4940.16518590101552547No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4830.16150767245040243No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC4800.16050451920536887No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGTGA4690.15682629064024584No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTGCCATGC4660.1558231373952123No Hit
CTCCTCGACACCGCCTCAGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA4540.15181052441507806No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG4380.1464603737748991No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4330.14478845169984317No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4300.1437852984548096No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4280.14311652962478724No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4270.14278214520977606No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4260.14244776079476487No Hit
ATGGCAACCACCGTGAACGCCCACCAGGTCTTGCCCAAGGTCTTACATAA4190.14010706988968658No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC4170.1394383010596642No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4160.13910391664465305No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4130.1381007633996195No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG4120.1377663789846083No Hit
CTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATC4080.13642884132456354No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4060.13576007249454117No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGACGAAGAAGG4010.13408815041948524No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4000.13375376600447406No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4000.13375376600447406No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3980.13308499717445169No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG3960.13241622834442932No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGCGGA3950.13208184392941813No Hit
GGTCAGGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC3950.13208184392941813No Hit
GGTCTGGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGC3930.13141307509939576No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3910.1307443062693734No Hit
GTCGTTGACATTGCTGCGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3880.12974115302433986No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT3860.1290723841943175No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAAGAGTCCTCTTATGT3830.1280692309492839No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG3750.12539415562919443No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3750.12539415562919443No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGCGG3740.12505977121418324No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT3740.12505977121418324No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC3740.12505977121418324No Hit
CGGTGGTTGCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3730.12472538679917207No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3730.12472538679917207No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3710.1240566179691497No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3670.12271908030910496No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG3660.12238469589409377No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3630.12138154264906022No Hit
GGGACTTAGTAGTCGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGA3580.11970962057400428No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3540.11837208291395954No Hit
GTATACATCTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTAAAC3500.1170345452539148No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT3500.1170345452539148No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3490.11670016083890361No Hit
CTCTTACAGTTAATGAAAAACGGAGATTAAAATTAATTATGCCTGCTAGG3490.11670016083890361No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCGTTTTTCA3490.11670016083890361No Hit
ATCTAAGCAGGCTTTCACTTTCTCACCAACTTACAAGGCCTTTCTGTGTA3460.11569700759387007No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3450.11536262317885888No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3440.1150282387638477No Hit
ATCCTAACCTTACCAAATATCTGCCCTTGGACAAAGGCATTAAACCATAT3440.1150282387638477No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA3420.11435946993382533No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC3390.11335631668879177No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT3390.11335631668879177No Hit
GGCCGATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG3350.11201877902874703No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC3350.11201877902874703No Hit
GCCTCCAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTA3310.11068124136870228No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3290.1100124725386799No Hit
CAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACAGTAGTTGT3290.1100124725386799No Hit
GAGAAACTGTGCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACT3290.1100124725386799No Hit
TAGCCGACTACTAAGTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCAC3290.1100124725386799No Hit
TCACCATACAGCTCTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA3280.10967808812366872No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA3250.10867493487863517No Hit
ACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGC3240.10834055046362398No Hit
GTCGATGAGATCTCGAATAGAAGGAAAGAAGTCAGAAGGCAAAAAAGAGA3240.10834055046362398No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC3220.10767178163360162No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3220.10767178163360162No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGT3170.1059998595585457No Hit
ACTAATTCTCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGC3160.10566547514353451No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT3160.10566547514353451No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCT3160.10566547514353451No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC3130.10466232189850096No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3120.10432793748348977No Hit
GTGGTTGCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3120.10432793748348977No Hit
TCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCG3110.10399355306847859No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC3100.1036591686534674No Hit
CATGGAGACCACCGTGAACGCCCACCAGGTCTTGCCCAAGGTCTTACATA3080.10299039982344504No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3070.10265601540843386No Hit
CTAATTCTCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGCC3030.10131847774838909No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3030.10131847774838909No Hit
GCTCTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3020.1009840933333779No Hit
GTAGAGTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG3020.1009840933333779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)