FastQCFastQC Report
Fri 1 Jul 2022
EGAF00002490636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495729
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA44670.9010971720435964No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTTTGACATACT34670.6993740531621108No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG27130.5472748215254706No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT22670.457306310504328No Hit
GGGTAAAGGTGCATGTATTGTTGACACAAAAAGGCCTTGTAAGTTGGCGA22460.4530701250078168No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG22240.44863221639242407No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT21920.44217707658821653No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21270.42906507386091997No Hit
CATGTATACAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC19400.3913428506300822No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC19170.386703218895808No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC18180.3667326301265409No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC17750.35805853601463705No Hit
AACATATTGTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAAT17600.35503268923141473No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA16650.3358689929376736No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG16640.33566726981879214No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC16450.3318345305600439No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG16300.32880868377682165No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCT16180.3263880063502438No Hit
TGCTTAGACTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACA15510.31287255738518427No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTGAATACAATA15310.30883809500755455No Hit
GCTTAGACTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT14980.3021812320844655No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAAT14680.29612953851802093No Hit
GGAACATATTGTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA14230.2870519981683541No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG14070.2838244282662503No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTGAAT13880.2799916890075021No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTGAATACAAT13880.2799916890075021No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTGAATACAATATGTT13850.27938651965085765No Hit
GTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAC13790.2781761809375687No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA13730.2769658422242798No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA13530.2729313798466501No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG13480.27192276425224265No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTCAAA13340.26909864058790184No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA12970.2616348851892869No Hit
ATATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG12900.2602228233571165No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA12740.25699525345501273No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG12660.2553814685039609No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT12210.24630392815429397No Hit
CCTCTATATTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC12190.24590048191653102No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC11730.23662121844798267No Hit
CTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAA11530.23258675607035298No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA11500.2319815867137085No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG11410.23016607864377514No Hit
TAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCATGGGATATGTAA11370.2293591861682492No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA11350.2289557399304862No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACT11320.22835057057384178No Hit
CTATATTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACT11040.22270232324516018No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATT10790.21765924527312303No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG10620.21422995225213776No Hit
CTTTACCACAGGAACATATTGTATTCAAACTCAAGCAATGTTTTCGGAAA10580.21342305977661183No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATA10520.2122127210633229No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT10400.20979204363674508No Hit
GACTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT10390.20959032051786358No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC10260.2069679199724043No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTA10150.2047489656647079No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT10120.20414379630806348No Hit
GTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATAC9810.19789037962273742No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAG9660.19486453283951513No Hit
TGATCTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTA9150.18457665377655938No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT9110.18376976130103342No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA9080.18316459194438897No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9060.18276114570662602No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAAT9050.1825594225877445No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT9040.18235769946886302No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9040.18235769946886302No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC9040.18235769946886302No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG9020.18195425323110007No Hit
TCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT8900.17953357580452223No Hit
CATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAA8880.17913012956675925No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG8640.1742887747136036No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTTTGTGTC8460.17065775857373686No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC8410.16964914297932943No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCAGACC8320.16783363490939607No Hit
ATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTG8270.16682501931498864No Hit
ATTGTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACC8240.16621984995834418No Hit
ACTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTA8190.16521123436393675No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG8170.16480778812617378No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8120.16379917253176635No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC8070.16279055693735892No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGATTT8010.16158021822407No Hit
ATATTGTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA7960.16057160262966258No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC7800.1573440327275588No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTG7710.15552852465762546No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA7610.15351129346881057No Hit
GTGCATGTATTGTTGACACAAAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7610.15351129346881057No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATATTTCCCTCTTG7550.15230095475552166No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTG7500.15129233916111423No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7460.15048544668558828No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATA7450.1502837235667068No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7370.14866993861565492No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTA7340.14806476925901046No Hit
GCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTTT7330.147863046140129No Hit
GAAATATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCC7220.14564409183243263No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7220.14564409183243263No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG7200.14524064559466968No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA7200.14524064559466968No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG6950.14019756762263252No Hit
GAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATC6870.13858378267158064No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG6810.13737344395829174No Hit
AATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCATGGGATATGTAAT6800.13717172083941026No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA6670.13454932029395092No Hit
CATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGC6580.13273381222401756No Hit
TGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC6540.1319269197484916No Hit
GGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAA6430.12970796544079527No Hit
ATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAA6380.12869934984638784No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC6300.12708556489533596No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC6240.12587522618204705No Hit
CAGGAACATATTGTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGT6170.12446316434987664No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG6150.12405971811211366No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT6090.12284937939882476No Hit
ACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCG6060.1222442100421803No Hit
CATCCAACGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT6060.1222442100421803No Hit
CTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCATGGGATATGTA6040.12184076380441734No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTGCATGTATTGTTGACAC6000.12103387132889139No Hit
GGTGCATGTATTGTTGACACAAAAAGGCCTTGTAAGTTGGCGAGAAAGTG5990.1208321482100099No Hit
TATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAA5970.12042870197224693No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG5940.11982353261560247No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG5930.11962180949672098No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC5790.11679768583238018No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA5780.1165959627134987No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC5750.11599079335685425No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC5730.11558734711909129No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA5680.11457873152468386No Hit
GCAAATCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGG5590.11276322345475048No Hit
GTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5540.11175460786034305No Hit
GACTGAATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT5540.11175460786034305No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATT5480.11054426914705413No Hit
TCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTAC5430.10953565355264672No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5420.10933393043376523No Hit
CACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACCAA5420.10933393043376523No Hit
ATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTGA5390.10872876107712076No Hit
GTACTTTACCACAGGAACATATTGTATTCAAACTCAAGCAATGTTTTCGG5370.1083253148393578No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC5350.10792186860159483No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5340.10772014548271333No Hit
GAATATGATAAAACGCCGCAGACACATCCAACGATAGCCAGGACAAATTG5290.10671152988830591No Hit
ACCCTGCACCGAACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTG5280.10650980676942443No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTT5220.1052994680561355No Hit
GCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC5220.1052994680561355No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT5190.10469429869949104No Hit
CCTCCAATCACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGT5150.10388740622396511No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5150.10388740622396511No Hit
TGTATACAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTT5120.10328223686732066No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC5100.10287879062955768No Hit
CAAAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGACTGTA5100.10287879062955768No Hit
TTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCATGG5100.10287879062955768No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT5100.10287879062955768No Hit
TCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTG5090.10267706751067619No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5090.10267706751067619No Hit
AGTTTGAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTAC5040.10166845191626876No Hit
ATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT5000.10086155944074282No Hit
GGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAA4990.10065983632186135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)