FastQCFastQC Report
Fri 1 Jul 2022
EGAF00002490637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495729
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA37970.765942682393001No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTTTGACATACT29020.5854004909940713No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG27370.5521161763786263No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG23790.4798992998190543No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT21570.4351167674273645No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21240.4284599045042755No Hit
GGGTAAAGGTGCATGTATTGTTGACACAAAAAGGCCTTGTAAGTTGGCGA20680.4171634098469123No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT19590.39517558988883034No Hit
AACATATTGTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAAT18030.36370678334331863No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17690.3568481973013481No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC16730.3374827778887255No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTGAATACAATA16550.3338517617488588No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG16310.3290104068957031No Hit
CATGTATACAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC16290.32860696065794015No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC16250.3278000681824142No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC15860.31993286654603625No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTCAAA15690.316503573525051No Hit
GGAACATATTGTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA15340.309443264364199No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG14590.29431403044808757No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA14540.2933054148536801No Hit
GTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAC14250.2874554444061171No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG14240.2872537212872356No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA14220.2868502750494726No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTGAATACAATATGTT14110.2846313207417763No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTGAAT13980.2820089201963169No Hit
CCTCTATATTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC13620.27474688791658347No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA13540.27313310296553156No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTGAATACAAT13370.26970380994454635No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG13260.2674848556368499No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA13100.26425728573474616No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA13010.2624417776648128No Hit
GCTTAGACTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT12550.25316251419626445No Hit
CTTTACCACAGGAACATATTGTATTCAAACTCAAGCAATGTTTTCGGAAA12490.2519521754829756No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATT12360.2493297749375163No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG12250.2471108206298199No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACT12240.24690909751093845No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT12200.24610220503541247No Hit
CTATATTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACT11900.24005051146896794No Hit
TGCTTAGACTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACA11820.23843672651791603No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAAT11620.23440226414028634No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT11610.23420054102140483No Hit
ATATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG11610.23420054102140483No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCT11300.2279471243360788No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA11070.22330749260180463No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC11020.2222988770073972No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG10510.21201099794444142No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA10480.21140582858779697No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATA10130.20434551942694495No Hit
ATATTGTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGA9830.19829382586050037No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTA9540.19244385541293732No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAAT9520.19204040917517434No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG9450.19062834734300393No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA9370.18901456239195205No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9250.1865938849653742No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG9210.18578699248984828No Hit
CTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAA9150.18457665377655938No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTTTGTGTC9100.18356803818215195No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT9060.18276114570662602No Hit
ATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTATTG8910.17973529892340373No Hit
TAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCATGGGATATGTAA8880.17913012956675925No Hit
GACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGATTT8820.17791979085347034No Hit
ATTGTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACC8800.17751634461570737No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAG8730.17610428278353696No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT8670.17489394407024805No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT8290.1672284655527516No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG8210.1656146806016997No Hit
CTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTG8180.16500951124505525No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCAGACC8150.16440434188841083No Hit
GACTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT8120.16379917253176635No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG8090.1631940031751219No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG8080.16299228005624042No Hit
CTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATA7980.16097504886742553No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7940.1601681563918996No Hit
GTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT7690.15512507841986245No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC7650.15431818594433652No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT7580.15290612411216611No Hit
GTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATAC7540.15209923163664016No Hit
TGATCTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTA7450.1502837235667068No Hit
TCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT7420.14967855421006235No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7220.14564409183243263No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG7180.1448371993569067No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7120.1436268606436178No Hit
GAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATC7080.14281996816809184No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6950.14019756762263252No Hit
GTGCATGTATTGTTGACACAAAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6920.13959239826598807No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTA6910.13939067514710657No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC6880.13878550579046214No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA6780.13676827460164726No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC6780.13676827460164726No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6750.1361631052450028No Hit
GAAATATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCC6720.13555793588835838No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC6710.13535621276947687No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC6680.13475104341283242No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA6520.13152347351072866No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATATTTCCCTCTTG6500.13112002727296568No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG6470.1305148579163212No Hit
GTATGTCAAAGAATTGTGGGTCTTTTGGGATTTGCTGCCCCTTTTACACA6430.12970796544079527No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG6360.12829590360862486No Hit
CAGGAACATATTGTATTCAAACTCAAGCAATGTTTTCGGAAACTGCCTGT6360.12829590360862486No Hit
GCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTC6290.12688384177645448No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC6090.12284937939882476No Hit
CATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAA6080.12264765627994327No Hit
ACTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTA6050.12204248692329882No Hit
GGTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAA5920.1194200863778395No Hit
TGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTC5860.11820974766455059No Hit
ATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAA5850.1180080245456691No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTG5830.11760457830790613No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG5810.11720113207014317No Hit
AATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCATGGGATATGTAAT5800.1169994089512617No Hit
GTACTTTACCACAGGAACATATTGTATTCAAACTCAAGCAATGTTTTCGG5780.1165959627134987No Hit
CCTCTTGTCCTCCAATTTGTCCTGGCTATCGTTGGATGTGTCTGCGGCGT5770.11639423959461721No Hit
GTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTG5720.11538562400020978No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC5660.11417528528692089No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC5640.1137718390491579No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG5600.11296494657363197No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCAC5570.11235977721698749No Hit
CATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGG5560.11215805409810602No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5540.11175460786034305No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT5440.10973737667152819No Hit
TCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTAC5410.10913220731488373No Hit
ATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGT5320.10731669924495037No Hit
CCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTT5290.10671152988830591No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5240.10570291429389848No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5240.10570291429389848No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5150.10388740622396511No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA5140.10368568310508362No Hit
GACCAGACCGTTGCCGGGCAACGGGGTAAAGGTGCATGTATTGTTGACAC5120.10328223686732066No Hit
AGTTTGAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTAC5060.10207189815403174No Hit
CAATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTC5050.10187017503515026No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT5040.10166845191626876No Hit
GGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATT5030.10146672879738727No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA5020.1012650056785058No Hit
ACCCTGCACCGAACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTG5020.1012650056785058No Hit
GTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTA5020.1012650056785058No Hit
GTCCCCAACCTCCAATCACTCACCAACCTCTTGTCCTCCAATTTGTCCTG4990.10065983632186135No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4980.10045811320297986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)