FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399039
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13320.3338019592069948No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12830.3215224577046354No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12620.3162598142036242No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA10930.27390806412405805No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10560.2646357874794193No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGCGTAAAA10270.2573683274065943No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10150.2543611025488737No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGATTGGGATAGAAC9120.22854908918677122No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9060.22704547675791087No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8870.22228403739985317No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGC8870.22228403739985317No Hit
ATTCTATGTTGGGGTGAATTGATGAATCTGGCCACCTGGGTGGGAAGTAA8420.2110069441834006No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8300.20799971932567995No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7810.19572021782332055No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7660.1919611867511697No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT7510.18820215567901885No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7410.1856961349642516No Hit
TTTCAGTACAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACAT7290.18268891010653096No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7220.18093469560619388No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6950.17416843967632237No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6670.16715158167497413No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6390.16013472367362588No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6380.15988412160214915No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG6340.15888171331624226No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6260.1568768967444285No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC6210.15562388638704489No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG6110.15311786567227764No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC6080.15236605945784748No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC5990.15011064081455697No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5970.14960943667160354No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5950.14910823252865008No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5890.14760462009978975No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5860.14685281388535956No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA5760.14434679317059235No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC5560.1393347517410579No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGGAGGGAGTTTG5540.13883354759810443No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGGCCAGTAAAGTTTCCCAC5510.13808174138367427No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGC5470.13707933309776738No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGATT5450.13657812895481394No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5260.13181668959675621No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5180.12981187302494243No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5170.1295612709534657No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5130.1285588626675588No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4990.12505043366688467No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4960.12429862745245451No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGC4960.12429862745245451No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4900.12279501502359418No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4860.1217926067376873No Hit
ATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA4820.1207901984517804No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4800.12028899430882696No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4750.11903598395144334No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4720.11828417773701318No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4720.11828417773701318No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4690.117532371522583No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4690.117532371522583No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4650.11652996323667612No Hit
GATTGGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4650.11652996323667612No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACCC4650.11652996323667612No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4640.1162793611651994No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4620.11577815702224593No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTATGTTGGGGTGAATTGATGA4610.11552755495076923No Hit
GTTGACATCTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGGAG4610.11552755495076923No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4590.11502635080781577No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4550.11402394252190888No Hit
TTGTTCACATCTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGG4550.11402394252190888No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4530.11352273837895543No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4500.11277093216452527No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCTAG4440.11126731973566492No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4420.1107661155927115No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4400.11026491144975806No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG4390.11001430937828131No Hit
ATACAGCACTCAGGCAAGCTATTCTATGTTGGGGTGAATTGATGAATCTG4380.1097637073068046No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4350.10901190109237444No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACATATCCC4290.1075082886635141No Hit
GCGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT4280.10725768659203735No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4260.10675648244908394No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACAAATCTT4240.10625527830613049No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4190.10500226794874687No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGGCGGCCGGAACGG4170.10450106380579342No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4140.10374925759136325No Hit
ACATAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG4120.10324805344840979No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4080.10224564516250292No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4070.10199504309102618No Hit
GGACAAATCTTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAG4060.10174444101954946No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4050.10149383894807273No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4050.10149383894807273No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4050.10149383894807273No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4040.10124323687659602No Hit
GTACAGAAAATTAAGCACTGTTTTCGAAAACTGCCTGTAAATAGACCTAT4030.1009926348051193No Hit
CTCATAAGGTGGGAAACTTTACTGGCCTTTATTCTTCTACTGTACCTGTC4030.1009926348051193No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC4010.10049143066216586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)