FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399039
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13630.3415706234227732No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13450.3370597861361922No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12000.3007224857720674No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10840.27165264548076756No Hit
CTATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATA9850.24684304040457197No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGCGTAAAA9480.23757076375993327No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9140.22905029332972465No Hit
ATTCTATGTTGGGGTGAATTGATGAATCTGGCCACCTGGGTGGGAAGTAA8970.22479005811462038No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8500.21301176075521439No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8330.20875152554011012No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGATTGGGATAGAAC8290.20774911725420322No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8230.2062455048253429No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8200.20549369861091274No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGC7910.19822623853808777No Hit
ATTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7730.19371540125150674No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT7710.1932141971085533No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7630.1912093805367395No Hit
TTTCAGTACAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACAT7090.17767686867699647No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6960.17441904174779907No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6700.1679033878894043No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6630.16614917338906723No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6390.16013472367362588No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGC6290.15762870295885867No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACC6220.1558744884585216No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA6200.15537328431556816No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC6190.15512268224409143No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6080.15236605945784748No Hit
CTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGGAGGGAGTTTG6050.15161425324341732No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6030.15111304910046386No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG6020.15086244702898713No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG5940.14885763045717337No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5870.1471034159568363No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC5740.1438455890276389No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5670.14209137452730183No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5460.13682873102629067No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5240.13131548545380275No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5240.13131548545380275No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5220.1308142813108493No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5170.1295612709534657No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5130.1285588626675588No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACATTCCACCAAGCTCTGCTAG5100.12780705645312865No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC5100.12780705645312865No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGGCCAGTAAAGTTTCCCAC5020.12580223988131486No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4950.12404802538097781No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC4900.12279501502359418No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGATT4830.12104080052325712No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGC4800.12028899430882696No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4740.11878538187996664No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4700.11778297359405973No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4670.11703116737962956No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4660.11678056530815284No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG4640.1162793611651994No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4600.11527695287929249No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCAGCTGCCGTTCCGGCCGCCCACG4540.11377334045043216No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4540.11377334045043216No Hit
GTTGACATCTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGGAG4520.11327213630747872No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4520.11327213630747872No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4520.11327213630747872No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4500.11277093216452527No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4490.11252033009304856No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4480.11226972802157183No Hit
ATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA4460.11176852387861838No Hit
GGATGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC4460.11176852387861838No Hit
GATTGGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4400.11026491144975806No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4380.1097637073068046No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGCCTTTATTCTTCTACTG4370.10951310523532787No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4350.10901190109237444No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAAACC4340.1087612990208977No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4310.10800949280646754No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4230.10600467623465375No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4170.10450106380579342No Hit
GTACAATATGTTCTTGCGGTAAAGTACCCCAACATCCAATTACATATCCC4160.1042504617343167No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTATGTTGGGGTGAATTGATGA4140.10374925759136325No Hit
ATACAGCACTCAGGCAAGCTATTCTATGTTGGGGTGAATTGATGAATCTG4130.10349865551988653No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA4120.10324805344840979No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4110.10299745137693309No Hit
GTACAGAAAATTAAGCACTGTTTTCGAAAACTGCCTGTAAATAGACCTAT4090.10249624723397963No Hit
TTGTTCACATCTATTAATAATGTCCTCCTGCAAATGAATGTGAGGAAAGG4080.10224564516250292No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4080.10224564516250292No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4060.10174444101954946No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4050.10149383894807273No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4040.10124323687659602No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4040.10124323687659602No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4030.1009926348051193No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4030.1009926348051193No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACCC4020.10074203273364257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)