FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247308
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8520.34450968023678974No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8470.3424879098128649No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7160.2895175247060346No Hit
CTATCAATAATATCCTCCTGTAAATGAATGTTAGGGAAGGAGGGAGTTTG6720.2717259449754961No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6660.26929982046678635No Hit
GTGCTGGTGAACGGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6550.2648519255341517No Hit
ACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACA6540.26444757144936676No Hit
GTTTTCGGAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAA6440.2604040306015171No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6410.25919096834716226No Hit
ATATCTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATC6150.24867776214275317No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5950.24059068044705387No Hit
TTCCTAAAACATTGCTTTAGTTTTTGTATAATATGTTCCTGTGGTAAAGT5820.23533407734484935No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA5600.2264382874795801No Hit
GTATAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5480.22158603846216055No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5320.2151163731056011No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5150.2082423536642567No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4880.19732479337506267No Hit
CTACCGCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGA4590.1855985249162987No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4570.18478981674672879No Hit
CTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTA4270.17265919420317985No Hit
GATGCAGAGGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCAG4090.16538082067705048No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3970.16052857165963091No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG3970.16052857165963091No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3940.159315509405276No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC3910.15810244715092112No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3830.15486761447264139No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC3810.15405890630307148No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT3790.15325019813350155No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3790.15325019813350155No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3790.15325019813350155No Hit
CTCCGAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3750.15163278179436168No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC3700.14961101137043686No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTTAGGGAAGGAGGGAGTTTG3670.14839794911608198No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA3650.14758924094651205No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3610.14597182460737218No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC3590.14516311643780225No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3580.1447587623530173No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT3520.1423326378443075No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG3520.1423326378443075No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3460.13990651333559773No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAC3370.13626732657253304No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3310.13384120206382324No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3300.1334368479790383No Hit
ATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT3290.1330324938942533No Hit
ATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAAGCGC3270.13222378572468338No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3260.13181943163989843No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3230.1306063693855435No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3220.13020201530075856No Hit
GCGGTAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCAAGAAC3180.1285845989616187No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3170.12818024487683374No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3170.12818024487683374No Hit
GGCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGG3140.12696718262247886No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT3130.12656282853769388No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3120.12615847445290893No Hit
GGATTAAAGACAGGTACGGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3080.12454105811376906No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT3050.12332799585941416No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3040.12292364177462921No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3000.12130622543548934No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3000.12130622543548934No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC2960.11968880909634949No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA2960.11968880909634949No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2960.11968880909634949No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2930.11847574684199459No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA2930.11847574684199459No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC2900.1172626845876397No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA2880.11645397641806977No Hit
GTGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA2850.11524091416371489No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT2830.11443220599414496No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA2820.11402785190935999No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2820.11402785190935999No Hit
ACTCACCCCAACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAA2820.11402785190935999No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG2820.11402785190935999No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC2780.11241043557022014No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT2730.1103886651462953No Hit
CCTTTACCCCGTTTCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2720.10998431106151035No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2710.10957995697672537No Hit
CCAAAAGACCGCCGTGAGGTGGGGTGAACCCTGGCCCGAATGCTCCCGCT2690.10877124880715545No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG2660.10755818655280056No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA2660.10755818655280056No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG2640.10674947838323064No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTTCCCGGCAACGGTCAGGTC2630.10634512429844566No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT2600.10513206204409077No Hit
ATTCAAGATCTCCTTGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA2590.10472770795930579No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC2590.10472770795930579No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCG2580.10432335387452084No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC2560.10351464570495092No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2540.10270593753538097No Hit
ACCAGATATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACA2540.10270593753538097No Hit
GGATGAAGAGGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCA2540.10270593753538097No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG2540.10270593753538097No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA2520.10189722936581105No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT2510.1014928752810261No Hit
AGGAAGGAGTTTGCCACTCAGGATTAAAGACAGGTACGGTAGAAGAATAA2510.1014928752810261No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC2500.10108852119624112No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA2500.10108852119624112No Hit
GGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAGATCCCA2500.10108852119624112No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA2490.10068416711145615No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG2490.10068416711145615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)