FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247308
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8920.36068384362818834No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8400.3396574312193702No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7560.30569168809743313No Hit
GTTTTCGGAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAA7000.2830478593494752No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6660.26929982046678635No Hit
CTATCAATAATATCCTCCTGTAAATGAATGTTAGGGAAGGAGGGAGTTTG6570.26566063370372167No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6290.25433871932974267No Hit
ATATCTGGTAAGGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATC5910.23897326410791403No Hit
GTATAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5680.22967312015785982No Hit
GTGCTGGTGAACGGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5430.2195642680382357No Hit
ACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACA5370.21713814352952596No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5070.205007520985977No Hit
TTCCTAAAACATTGCTTTAGTTTTTGTATAATATGTTCCTGTGGTAAAGT4860.19651608520549277No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4860.19651608520549277No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA4850.1961117311207078No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4630.18721594125543856No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4530.18317240040758892No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4520.18276804632280397No Hit
CTACCGCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGA4340.17548967279667457No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4220.17063742377925503No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA4120.16659388293140537No Hit
CTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTA4090.16538082067705048No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4070.16457211250748055No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC3940.159315509405276No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3920.1585068012357061No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3910.15810244715092112No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3820.15446326038785643No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3810.15405890630307148No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC3630.1467805327769421No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3510.14192828375952254No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3460.13990651333559773No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAC3430.1386934510812428No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT3430.1386934510812428No Hit
GATGCAGAGGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCAG3410.1378847429116729No Hit
CTCCGAAAGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3380.136671680657318No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3380.136671680657318No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3370.13626732657253304No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3360.13586297248774806No Hit
ATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT3340.13505426431817813No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3340.13505426431817813No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3330.13464991023339318No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3300.1334368479790383No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG3300.1334368479790383No Hit
ATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAAGCGC3300.1334368479790383No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTTAGGGAAGGAGGGAGTTTG3280.13262813980946836No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA3260.13181943163989843No Hit
GTGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA3240.1310107234703285No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3230.1306063693855435No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3210.1297976612159736No Hit
GGATTAAAGACAGGTACGGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3180.1285845989616187No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3180.1285845989616187No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3170.12818024487683374No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT3110.12575412036812397No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC3080.12454105811376906No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3030.12251928768984424No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3010.12171057952027431No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCTACCACG2910.11766703867242467No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACAT2890.11685833050285473No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC2880.11645397641806977No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA2880.11645397641806977No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG2880.11645397641806977No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA2870.11604962233328481No Hit
CCTTTACCCCGTTTCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2870.11604962233328481No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA2860.11564526824849986No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG2820.11402785190935999No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2820.11402785190935999No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2810.11362349782457504No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2810.11362349782457504No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTTCCCGGCAACGGTCAGGTC2780.11241043557022014No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2770.11200608148543517No Hit
GCGGTAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCAAGAAC2740.11079301923108027No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG2720.10998431106151035No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT2720.10998431106151035No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG2710.10957995697672537No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG2710.10957995697672537No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA2700.10917560289194041No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA2700.10917560289194041No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCG2690.10877124880715545No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG2680.10836689472237049No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG2650.10715383246801559No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT2630.10634512429844566No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT2630.10634512429844566No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC2570.10391899978973587No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA2570.10391899978973587No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC2550.10311029162016595No Hit
GAAAAACGGAGATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCT2540.10270593753538097No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA2520.10189722936581105No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT2520.10189722936581105No Hit
CCAAAAGACCGCCGTGAGGTGGGGTGAACCCTGGCCCGAATGCTCCCGCT2510.1014928752810261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)