FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539868
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA17150.3176702453192262No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15620.2893299843665489No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15530.28766291019286194No Hit
GTATAATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCC13750.2546918876466099No Hit
ATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGAA12910.23913252869219884No Hit
GGACCATATTATACTGAAACTCAAGGAATGTTTTCGGAAACTGCCTGTAA11680.2163491816518112No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC11140.20634673660968977No Hit
ATATTATACTGAAACTCAAGGAATGTTTTCGGAAACTGCCTGTAAATAGG11120.20597627568220378No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGATGAGAGTCCAA10780.19967843991494216No Hit
AATATGTTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCCATGA10590.1961590611038254No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10540.19523290878511043No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9910.18356338956930213No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG9610.17800647565701244No Hit
TGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTT9240.17115294849852186No Hit
ATCCTAACCTTACCAAATACTTGCCCTTGGATAAAGGCATTAAACCTTAT9130.16911541339734898No Hit
CATGTATACACTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9120.16893018293360598No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTGCCACAG9050.16763356968740506No Hit
GTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAA8980.16633695644120414No Hit
GACCCACAATTCTTTGGCATACTTTCCAATCAATAGGCCTATTTACAGGC8880.16448465180377425No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA8580.1589277378914846No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA8540.15818681603651263No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTC8510.15763112464528367No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8430.15614928093533975No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC8410.15577882000785379No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8240.15262990212422295No Hit
TTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGTGTCC8230.15244467166047995No Hit
TCTGTAGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAATATTCG8180.15151851934176502No Hit
CTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA8160.15114805841427906No Hit
CAAGATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTG7880.14596160542947537No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7860.14559114450198937No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7730.1431831484733305No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7630.14133084383590064No Hit
CTGTAGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAATATTCGA7580.14040469151718568No Hit
GATAAGATAGGGGCATTTGGTGGTCTGTAAGCTGGAGGAGTGCGAATCCA7450.13799669548852683No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT7440.13781146502478384No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACT7280.13484777760489602No Hit
GACAAGAAATGTGAAACCACAATAGTTGTCTGATTTTTAGGCCCATATTA7280.13484777760489602No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7100.1315136292575222No Hit
GTACACCACCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6870.12725332859143346No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6850.12688286766394746No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCAGCTTACAGACCACCAAATGCC6850.12688286766394746No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCATCCATGTCAACGACCGACCT6840.1266976372002045No Hit
GCTTAGAGTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT6790.12577148488148956No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6720.12447487163528863No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6670.12354871931657369No Hit
ACACCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGG6590.12206687560662978No Hit
GTATAATATGGTCCTGTGGCAAAGTACCCCAACTTCCAATTACATATCCC6380.118177035868027No Hit
ATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGCACTTG6120.11336104381070929No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6010.1113235087095364No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6010.1113235087095364No Hit
TTGTAGGTCTTTTGGGCTTTGCTGCCCCTTTTACACAATGTGGCTATCCT5970.11058258685456444No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5970.11058258685456444No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5930.1098416649995925No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5920.1096564345358495No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5920.1096564345358495No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA5740.10632228618847571No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5720.10595182526098972No Hit
TTATTAATAGATGTGAACAATATGTGGGCCCTCTTACAACTAATGAAAAA5680.10521090340601777No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5670.10502567294227477No Hit
CATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5650.1046552120147888No Hit
CGTATAAAGAATTTGGAGCTTCTGTAGAGTTACTCTCTTTTTTGCCTTCT5640.10446998155104581No Hit
CTCTAAGGCCTCCCGATGCAGAGCAGCGGCGGTGTCGAGGAGATCTCGAA5580.10335859876858787No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTTC5550.10280290737735892No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5530.10243244644987293No Hit
CAACACCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTT5520.10224721598612996No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC5520.10224721598612996No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG5500.10187675505864396No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA5420.10039491134870004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)