FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455381
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20760.45588199771180615No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17510.38451318785807925No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15210.3340060301154418No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC11450.25143780702313007No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10770.2365052560383503No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG10200.22398826477169667No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9440.20729894308282515No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9300.2042245943506646No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9200.2020286309705499No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9190.20180903463253846No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA9000.1976367042103206No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8340.18314334590156375No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT8290.1820453642115064No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8270.18160617153548347No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA7970.17501828139513947No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACATTGCTGAGAGTCCAA7880.17304191435303626No Hit
CCCTAGCAGCCATGGGAATGAGGTGTATTTCCGAGAGAGGACAACCGAGT7630.16755200590274955No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7550.16579523519865783No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAAT7390.16228169379047436No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCCATTCG7380.1620620974524629No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7340.16118371210041701No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7080.15547420731211886No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7070.1552546109741074No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7020.15415662928405005No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCTTTGACATACT6770.14866672083376337No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6280.13790650027120147No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG6260.13746730759517856No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA6260.13746730759517856No Hit
CACATATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAG6100.13395376618699506No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6070.13329497717296065No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6050.13285578449693772No Hit
GATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCC6020.1321969954829033No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5930.13022062844080012No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5850.12846385773670838No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5840.1282442613986969No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5830.12802466506068544No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5640.12385233463846758No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5620.12341314196244464No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5590.12275435294841024No Hit
CACAAATTGTTGACATCTATTAATAATGTCCTCCTGTAAATGAATGTTAG5550.12187596759636436No Hit
GATTAAGATTAATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATAT5550.12187596759636436No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5430.11924081154022677No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG5420.11902121520221529No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5300.11638605914607769No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5290.11616646280806621No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5290.11616646280806621No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5240.11506848111800887No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5160.11331171041391713No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5130.11265292139988274No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5100.11199413238584834No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5100.11199413238584834No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5080.1115549397098254No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5060.11111574703380246No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTGCCAGC5060.11111574703380246No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGCAG5030.11045695801976806No Hit
AATTATGCCTGCTAGGTTTTATCCTAACCTTACCAAATATTTGCCCTTGG4980.10935897632971073No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4970.10913937999169926No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4900.10760220562561898No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4890.10738260928760752No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4880.10716301294959604No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4810.10562583858351579No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4790.10518664590749285No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT4740.10408866421743551No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4740.10408866421743551No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4660.10233189351334378No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4650.1021122971753323No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTTCACCAAGCTCTGCTAG4590.1007947191472635No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4590.1007947191472635No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4580.10057512280925204No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA4580.10057512280925204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)