FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416487
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG23670.5683250617666338No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18560.4456321565859198No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT17380.4172999397340133No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14840.35631364244262126No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG12720.3054116935222468No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12540.3010898299346678No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12500.30012941580409475No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11650.2797206155294163No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11540.2770794766703403No Hit
CCCATCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCT11520.2765992696050537No Hit
GGGTAAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAGCCAC10700.2569107799283051No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10550.25330922693865593No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC10320.2477868456878606No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT9560.22953897720697164No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9490.2278582524784687No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9190.22065514649917045No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9000.2160931793789482No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT8910.21393224758515872No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8790.21105100519343942No Hit
GGATCCTGGGTCTTCCAGATTACTTCCCACCCAGGTGGCCAGATTCATCA8760.2103306945955096No Hit
CCCAGGATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCT8720.20937028046493647No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8450.20288748508356805No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC8110.19472396497369665No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCACAA7810.18752085899439838No Hit
ATGTAATTGGATGTTGGGGTACTTTACCACAAGAACATATTGTACTAAAA7470.179357338884527No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7440.17863702828659717No Hit
GCCTCACCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAA7360.176716200025451No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7330.17599588942752115No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGTGAGAGGACAACAGAGT6940.1666318516544334No Hit
GTTAGTATCCCTTGGACGCATAAGGTGGGAAACTTTACTGGGCTTTATTC6940.1666318516544334No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG6800.16327040219742753No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6780.162790195132141No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6760.16230998806685443No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6610.1587084350772053No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6380.15318605382640993No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA6360.1527058467611234No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6350.15246574322848014No Hit
GCCTTACCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAA6240.1498246043694041No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6210.14910429377147427No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG6110.1467032584450415No Hit
GTCCCGTGCTGGTAGTTGAAGTTCCTGGAAGTAGAGGACAAACGGGCAAC6110.1467032584450415No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC6090.14622305137975494No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA6090.14622305137975494No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT6040.1450225337165386No Hit
ATGTTAATATGGGCTTAAAAATCAGACAACTACTGTGGTTTCACGTTTCC5900.1416610842595327No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5820.13974025599838652No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5750.13805953126988357No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA5670.13613870300873737No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5600.13445797828023445No Hit
GGGTGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAGCCAC5580.1339777712149479No Hit
GTTAATATGGGCTTAAAAATCAGACAACTACTGTGGTTTCACGTTTCCTG5480.13157673588851512No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5460.1310965288232286No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5460.1310965288232286No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5430.13037621822529874No Hit
GGATGATGGGTCTTCCAGATTACTTCCCACCCAGGTGGCCAGATTCATCA5420.13013611469265546No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5390.12941580409472567No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5370.1289355970294391No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5360.12869549349679582No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5320.12773507936622272No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5320.12773507936622272No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5310.12749497583357944No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG5260.12629445817036306No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5080.1219725945827841No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA5050.12125228398485427No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT5000.12005176632163789No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACAAAATGCC4970.11933145572370807No Hit
ATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGAT4970.11933145572370807No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4950.1188512486584215No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4940.11861114512577824No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGACGAGAAGGCACAG4940.11861114512577824No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4920.11813093806049169No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4920.11813093806049169No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4870.1169304203972753No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4800.11524969566877237No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT4730.11356897094026944No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTT4680.11236845327705305No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4650.11164814267912325No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4570.10972731441797703No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGT4570.10972731441797703No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4560.10948721088533375No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4550.10924710735269048No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4510.10828669322211738No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4400.10564555436304135No Hit
GGATACTAACAGTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4370.1049252437651115No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTG4320.10372472610189513No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT4310.10348462256925185No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG4300.10324451903660858No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCAGGCGTTGATGGCC4300.10324451903660858No Hit
CTCTAAGGCCTCCCGATACAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA4290.10300441550396532No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4290.10300441550396532No Hit
GATGATGGGTCTTCCAGATTACTTCCCACCCAGGTGGCCAGATTCATCAA4270.10252420843867875No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAACGTGAAACCACA4220.10132369077546238No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCGTCTGCCGGTCCGTGTG4170.10012317311224601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)