FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416487
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG22410.5380720166535811No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18580.44611236365120643No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT15850.3805640992395921No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15780.3788833745110892No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG12920.3102137641751123No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11870.28500289324756833No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11710.28116123672527593No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA11620.2790003049314864No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11110.2667550247666794No Hit
CCCATCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCT11080.2660347141687496No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9720.23338063372926407No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9690.23266032313133422No Hit
GGGTAAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAGCCAC9440.22665773481525234No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9230.22161556062974355No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT9200.22089525003181373No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9200.22089525003181373No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTT9100.21849421470538097No Hit
GGATCCTGGGTCTTCCAGATTACTTCCCACCCAGGTGGCCAGATTCATCA8870.21297183345458565No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8590.20624893454057389No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCACAA8580.20600883100793063No Hit
GCCTCACCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAA8260.1983255179633458No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC7830.18800106605968495No Hit
CCCAGGATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCT7740.18584013426589546No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7500.18007764948245683No Hit
ATGTAATTGGATGTTGGGGTACTTTACCACAAGAACATATTGTACTAAAA7410.17791671768866735No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7360.176716200025451No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG7180.172394336437872No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA7030.16879278344822285No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6760.16230998806685443No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6740.16182978100156786No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6640.15942874567513513No Hit
GTTAGTATCCCTTGGACGCATAAGGTGGGAAACTTTACTGGGCTTTATTC6620.15894853860984856No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCGTCTGCCGGTCCGTGTG6610.1587084350772053No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6550.15726781388134561No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA6540.15702771034870236No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6510.15630739975077254No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG6440.1546266750222696No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6420.15414646795698306No Hit
GCCTTACCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAA6350.15246574322848014No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGTGAGAGGACAACAGAGT6180.14838398317354443No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6150.1476636725756146No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC5870.1409407736616029No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5860.1407006701289596No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5810.1395001524657432No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5790.13901994540045667No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5740.1378194277372403No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5670.13613870300873737No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTGGGGTTCAAATGTATA5660.1358985994760941No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5650.1356584959434508No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT5550.13325746061701807No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG5510.13229704648644497No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5510.13229704648644497No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5500.1320569429538017No Hit
GTCCCGTGCTGGTAGTTGAAGTTCCTGGAAGTAGAGGACAAACGGGCAAC5500.1320569429538017No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5460.1310965288232286No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5460.1310965288232286No Hit
ATGTTAATATGGGCTTAAAAATCAGACAACTACTGTGGTTTCACGTTTCC5440.13061632175794202No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5320.12773507936622272No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5290.1270147687682929No Hit
GGGTGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAGCCAC5290.1270147687682929No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5280.1267746652356496No Hit
GTTAATATGGGCTTAAAAATCAGACAACTACTGTGGTTTCACGTTTCCTG5220.12533404403978995No Hit
ATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGAT5200.1248538369745034No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5140.12341321577864375No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5080.1219725945827841No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA5070.12173249105014083No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5030.12077207691956772No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4910.11789083452784842No Hit
GTTTGGGACTCTACCGTCCCCTTCTGCATCTGCCGTTCCAGCCAACCACG4890.11741062746256185No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4840.11621010979934547No Hit
CATTTGAACCCCAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4830.1159700062667022No Hit
GTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC4830.1159700062667022No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACAAAATGCC4830.1159700062667022No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4780.11476948860348582No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4780.11476948860348582No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTGTCTGCCAAGTGTTTGCTG4770.11452938507084255No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4610.11068772854855013No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4590.11020752148326357No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAAT4530.10876690028740392No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTG4470.10732627909154427No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTT4430.10636586496097115No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4400.10564555436304135No Hit
CTCTAAGGCCTCCCGATACAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA4390.10540545083039807No Hit
GGATACTAACAGTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4380.1051653472977548No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT4360.10468514023246824No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAACGTGAAACCACA4310.10348462256925185No Hit
ATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATATGTT4290.10300441550396532No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGT4280.10276431197132205No Hit
GATTAACTGCATGTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGC4270.10252420843867875No Hit
GGATGATGGGTCTTCCAGATTACTTCCCACCCAGGTGGCCAGATTCATCA4270.10252420843867875No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4250.1020440013733922No Hit
GTTTTATTGGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT4230.10156379430810566No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4200.10084348371017582No Hit
AGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4190.10060338017753255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)