FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255941
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13150.5137902876053466No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9570.3739143005614575No Hit
CTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7140.2789705439925608No Hit
ATCCTAACCTTACCAAATATTTGCCATTGGACAAAGGCATTAAACCATAT7100.27740768380212627No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6530.25513692608843447No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA6110.23872689408887204No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTCTGTGGGTCACCATA5640.2203632868512665No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT5370.20981398056583353No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA5100.19926467428040057No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT4860.18988751313779348No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4470.17464962628105696No Hit
CCCTAGCAGCCATGGAAATGAGGTGTATTTCCGAGAGAGGACAACAGAGT4420.17269605104301383No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC4410.17230533599540518No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCGATGTCAACGACCGACCT4370.17074247580497068No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC4320.16878890056692752No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG4120.16097459961475497No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA4090.15980245447192906No Hit
GGATAATATGGTTTAATGCCTTTGTCCAATGGCAAATATTTGGTAAGGTT4020.15706744913866869No Hit
CTCTTATATAGAATGCCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT3970.15511387390062553No Hit
ATATAAGAGAGAAACTACACGCAGTGCCTCATTCTGTGGGTCACCATATT3950.15433244380540828No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA3890.1519881535197565No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA3880.15159743847214788No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3720.14534599771040982No Hit
ATTTACATACTCTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACA3710.1449552826628012No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG3560.13909455694867176No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA3460.13518740647258548No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG3360.1312802559964992No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT3350.13088954094889058No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA3340.13049882590128195No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3330.13010811085367333No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3280.12815453561563017No Hit
GGACATCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGATCCG3280.12815453561563017No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3260.1273731055204129No Hit
CCTAGTACCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAA3240.12659167542519564No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3200.12502881523476114No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3170.12385667009193525No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTACTGTGGTTTCACATTTCC3150.12307523999671799No Hit
CTCTAAGGCCTCCCGATACAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA3120.12190309485389211No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3120.12190309485389211No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGATCCGTCTCG3080.1203402346634576No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3070.11994951961584897No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3070.11994951961584897No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3060.11955880456824035No Hit
GCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAGCACTCAGGCA3060.11955880456824035No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3040.11877737447302308No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3040.11877737447302308No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCATTGGACAAAGGCATTAAAC3000.11721451428258857No Hit
ATTTCAATCTCCTTTTTTCATTAACTGTAAGAGGGCCCACATATTGTTGA2980.1164330841873713No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2970.11604236913976267No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAATGGCAAATATTTGGTA2940.11487022399693679No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC2920.11408879390171953No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT2910.1136980788541109No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG2910.1136980788541109No Hit
GGATGGGGCTTGGCTATCGGCCATCGCCGCATGCGTGGAACCTTTGTGGC2880.11252593371128503No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2870.11213521866367639No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC2850.11135378856845914No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC2840.11096307352085051No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG2840.11096307352085051No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGATTTTTAGTACAATA2820.11018164342563325No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2810.10979092837802462No Hit
TTTACATACTCTGTGGAAGGCGGGCATTCTATATAAGAGAGAAACTACAC2800.109400213330416No Hit
ATTGACAACCGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT2800.109400213330416No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG2780.10861878323519875No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA2770.10822806818759012No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG2760.10783735313998148No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG2740.10705592304476423No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC2720.10627449294954698No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG2710.10588377790193834No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2700.1054930628543297No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2700.1054930628543297No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG2690.10510234780672106No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA2680.10471163275911244No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC2660.10393020266389519No Hit
GCACCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT2650.10353948761628656No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA2650.10353948761628656No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG2620.10236734247346067No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA2610.10197662742585205No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC2600.10158591237824342No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG2600.10158591237824342No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC2600.10158591237824342No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCTCCTCACCATACAG2560.10002305218780891No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)