FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261810
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14720.562239792215729No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9540.3643863870745961No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9020.34452465528436654No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8870.33879530957564646No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8400.3208433596883236No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7800.29792597685344335No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGT7320.27959207058553914No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6820.26049425155647227No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA6610.2524731675642642No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6160.23528513043810398No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6070.23184752301287195No Hit
GGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA6060.2314655666322906No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6010.2295557847293839No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5990.2287918719682212No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5900.22535426454298918No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5780.22077078797601313No Hit
TTTATATAGAATGCCAGCCTTCCACAGGGTATGTAAATAATGCCTAGTTT5600.21389557312554908No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5530.21122187846147972No Hit
ATATTTCCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5440.20778427103624766No Hit
TCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5190.1982353615217142No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5090.19441579771590084No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5090.19441579771590084No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4870.18601275734311143No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4700.17951949887322868No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4670.17837362973148468No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA4660.17799167335090332No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4560.17417210954508994No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4530.17302624040334594No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4400.16806080745578855No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4190.16003972346358047No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4150.1585118979412551No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4130.15774798518009245No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4110.15698407241892975No Hit
CTCGAAAATACACCTCCTTCCCATGGCTGCTCGGGTGTGCTGCCAACTGG4100.15660211603834842No Hit
CTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4050.15469233413544173No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA3980.15201863947137237No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT3980.15201863947137237No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA3900.14896298842672168No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA3830.1462892937626523No Hit
CTTATATAAGAGCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA3830.1462892937626523No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT3820.14590733738207096No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA3820.14590733738207096No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3810.14552538100148965No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT3810.14552538100148965No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3770.14399755547916426No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3750.1432336427180016No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3720.1420877735762576No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3710.14170581719567626No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG3670.1401779916733509No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3670.1401779916733509No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3660.13979603529276957No Hit
ATTTCCGAGAGAGGACAACAGAGTTGTCAGTCCCGATAAGTTTTGCTCCA3640.13903212253160688No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA3590.1371223406287002No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA3590.1371223406287002No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3590.1371223406287002No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3570.13635842786753752No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATTAGGGTTCAAATGTATA3560.13597647148695619No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3480.13292082044230547No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3460.13215690768114283No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3430.1310110385393988No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC3420.13062908215881747No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA3420.13062908215881747No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC3390.12948321301707344No Hit
TTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC3360.12833734387532944No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC3330.12719147473358544No Hit
ATTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA3310.12642756197242275No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3310.12642756197242275No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3260.12451778006951607No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3260.12451778006951607No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3250.12413582368893472No Hit
ATTTTCGAGAGAGGACAACAGAGTTGTCAGTCCCGATAAGTTTTGCTCCA3240.12375386730835339No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3240.12375386730835339No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC3210.12260799816660939No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3180.12146212902486536No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3110.118788434360796No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3110.118788434360796No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA3090.11802452159963331No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT3080.11764256521905199No Hit
GAATATGGTGACCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTTTTATA3080.11764256521905199No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3020.11535082693556395No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAAT3020.11535082693556395No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA2990.11420495779381995No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG2980.1138230014132386No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTC2960.11305908865207592No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT2950.11267713227149459No Hit
GCTTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT2950.11267713227149459No Hit
CTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA2930.11191321951033192No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG2900.1107673503685879No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG2900.1107673503685879No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA2900.1107673503685879No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC2880.11000343760742524No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG2880.11000343760742524No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT2860.10923952484626256No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCT2850.10885756846568122No Hit
GTAGAAGAGTAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT2850.10885756846568122No Hit
GCCATGGGAAGGAGGTGTATTTTCGAGAGAGGACAACAGAGTTGTCAGTC2840.10847561208509988No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG2840.10847561208509988No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG2810.10732974294335587No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC2810.10732974294335587No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2800.10694778656277454No Hit
GGATTAAAGACAGGTACAGTAGAAGAGTAAAGCCCAGTAAAGTTTCCCAC2800.10694778656277454No Hit
GTATACATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAAC2780.10618387380161186No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT2780.10618387380161186No Hit
GGGAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT2760.10541996104044918No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2740.10465604827928651No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG2720.10389213551812383No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2720.10389213551812383No Hit
ATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG2700.10312822275696115No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG2700.10312822275696115No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT2690.1027462663763798No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT2690.1027462663763798No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG2690.1027462663763798No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG2690.1027462663763798No Hit
GGGTTCACCCCGCCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA2690.1027462663763798No Hit
CCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGCAGCGCT2680.10236430999579849No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC2680.10236430999579849No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC2650.10121844085405447No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA2650.10121844085405447No Hit
AAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATA2640.10083648447347313No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTG2620.10007257171231045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)