FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002490658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683084
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA39380.5765030362298048No Hit
GTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA23370.3421248338418116No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT23290.3409536748042701No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT20330.2976207904152344No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT18690.2736120301456336No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17320.2535559316277354No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT16000.23423180750830058No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG15800.23130390991444685No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA15320.2242769556891978No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC14920.2184211605014903No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT14810.21681081682487072No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTGAAACATTCTTTGATTTTCTGT14490.21212618067470473No Hit
ATATTAGTGTTAACATAACTAACTACTAGGTCCCTGGATATTGGATCTTC13810.20217132885560193No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT13460.19704750806635787No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA13220.19353403095373337No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13180.19294845143496261No Hit
GTATGGTGAGGTGAACAATGCTCAGGAGACTCTAAGGCTTCCCGATATAG12660.18533591769094285No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT12550.1837255740143233No Hit
GTTTATACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA12300.18006570202200609No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT12300.18006570202200609No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG12280.1797729122626207No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC12250.17933372762354263No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG11400.16689016284966415No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC11230.16440144989488847No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT11110.16264471133857622No Hit
CTGTATAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGG10940.1601559983838005No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA10940.1601559983838005No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT10910.15971681374472246No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATAATTAATCT10850.15883844446656634No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT10740.15722810078994678No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG10410.15239706976008807No Hit
ATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTT10140.14844440800838551No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA10050.1471268540911513No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT9730.1424422179409853No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT9730.1424422179409853No Hit
GGATAACCACATTGCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT9660.14141745378313647No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC9650.1412710589034438No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9630.14097826914405842No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC9540.13966071522682422No Hit
GCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9540.13966071522682422No Hit
CATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAAGGGGCAGCA9510.13922153058774617No Hit
GGATAATAAGGTTTAATGCCCTTATCCAATGGCAAATATTTGGTAACCTT9320.1364400278735851No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC9230.1351224739563509No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA9040.13234097124218983No Hit
TAATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG8980.1314626019640337No Hit
GGATAAGGGCATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8960.13116981220464835No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA8770.12838830949048727No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8720.12765633509202381No Hit
CTCACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT8650.12663157093417501No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC8460.12385006822001393No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT8200.12004380134800405No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG8180.11975101158861869No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8100.11857985255107717No Hit
ACCTAGTAGTTAGTTATGTTAACACTAATATGGGCCTAAAGTTTAGGCAA7870.11521277031814536No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7830.11462719079937461No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG7800.11418800616029653No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7770.11374882152121847No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7690.11257766248367698No Hit
CCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGA7680.11243126760398428No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7640.11184568808521353No Hit
GTATTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAA7620.11155289832582817No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGCATTAAACCTTAT7580.11096731880705742No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT7570.11082092392736471No Hit
CCTAATACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC7550.11052813416797935No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAAGGGGC7540.11038173928828664No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA7500.1097961597695159No Hit
TTGACAGACTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTGAAAC7450.10906418537105246No Hit
ACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGG7440.10891779049135979No Hit
ATCCAATATCCAGGGACCTAGTAGTTAGTTATGTTAACACTAATATGGGC7430.10877139561166707No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7430.10877139561166707No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7420.1086250007319744No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7410.10847860585228171No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG7380.10803942121320365No Hit
CTACTAGGTCCCTGGATATTGGATCTTCCAAATTACCACCCACCCAGGTG7360.10774663145381826No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT7260.1062826826568914No Hit
ACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCA7230.10584349801781333No Hit
CTCCGAAAGACACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGA7180.10511152361934989No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7180.10511152361934989No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC7170.1049651287396572No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT7130.10437954922088645No Hit
CACCAAATACTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGT6970.10203723114580343No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAA6970.10203723114580343No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC6900.10101246698795462No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6890.10086607210826194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)