FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579236
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113611.9613767100111181No Hit
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA32570.5622923989531037No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT21800.37635782306348364No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT16470.2843400617364943No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCGAGTACAAAGATC12870.22218922857004747No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTCTGCATTTCAT12000.2071694438881561No Hit
CACACAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGACCTATT11520.1988826661326299No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11520.1988826661326299No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGCTGACACACT10810.18662514070258066No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT10590.18282703423129779No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9850.17005158519152816No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9840.169878943988288No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGG9760.16849781436236697No Hit
CTTTCCAATCAATAGGTCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG9390.16211008984248215No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA9390.16211008984248215No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC9190.15865726577767955No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT8930.15416859449343617No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG8820.15226954125779474No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA8160.14087522184394616No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8130.14035729823422577No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT8120.14018465703098565No Hit
CACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT8090.13966673342126523No Hit
TTTTTTGTGTGATGTGTTCTTGTGGCAATGACCCATAACATCCAATGACA7970.1375950389823837No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC7920.13673183296618305No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT7890.13621390935646266No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT7860.13569598574674227No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7560.13051674964953836No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7470.1289629788203772No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT7260.12533751355233447No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC7130.12309317791021276No Hit
GCTACGTGTTGTTTCTCTTTTATATAATATACCCGCCTGCCATAGAGTGT7130.12309317791021276No Hit
CTTTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT7030.12136676587781146No Hit
GCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGG6970.12033091865837069No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGG6970.12033091865837069No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT6880.11877714782920952No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA6830.11791394181300885No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGGTGTAAATGTATATT6770.11687809459356809No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT6730.11618752978060755No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG6630.11446111774820625No Hit
GGGTTACTCTCTGCATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT6390.11031772887044314No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6350.1096271640574826No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6310.10893659924452209No Hit
GTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6290.10859131683804184No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6270.10824603443156157No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA6010.10375736314731819No Hit
CTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACACC5930.10237623352139714No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG5920.10220359231815701No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5880.1015130275051965No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5820.10047718028575571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)