FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495180
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT27340.552122460519407No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT24070.48608586776525703No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG17370.3507815339876409No Hit
TTCTTCTACTGTCCCTATCTTTAATTCTGAATGGCAAACCCCTTCTTTTC16070.3245284542994467No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG14840.29968900197907833No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT14690.29665980047659435No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA13570.2740417625913809No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT13330.2691950401874066No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATACCCCATGAA13100.26455026455026454No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA12960.2617230098146129No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA11510.23244072862393475No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT11310.22840179328728946No Hit
GGATCATATTGTACAAAAAATCAAAAACTGTTTTAGAAAACTTCCTGTCA11240.22698816591946364No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10740.2168908275778505No Hit
CTTTAATTCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG10220.2063895957025728No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT10000.20194676683226304No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9860.19911951209661133No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9390.18962801405549498No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9240.18659881255301103No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT8920.1801365160143786No Hit
GTCCCTATCTTTAATTCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCA8810.17791510157922372No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT8610.17387616624257846No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG8280.1672119229371138No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTG8060.162769094066804No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC7680.155095116927178No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA7510.15166202189102954No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7500.15146007512419726No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT7440.15024839452320368No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTCCCTATC7430.15004644775637144No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7190.1451997253523971No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA7180.14499777858556484No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT7010.14156468354941637No Hit
CTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGG7000.14136273678258413No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG6970.14075689648208733No Hit
GAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCA6960.14055494971525506No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG6940.14015105618159054No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG6870.1387374288137647No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG6840.1381315885132679No Hit
CTGTAAATGAAAAAAGAAGACTGAAATTAATTATGCCTGCTAGGTTTTAT6760.1365160143786098No Hit
ATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT6680.1349004402439517No Hit
CTTCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTA6630.13389070640979037No Hit
GTTTTATCCTAACAGCACAAAATATTTGCCTTTAGACAAAGGGATTAAAC6530.13187123874146775No Hit
CTTCTACTGTCCCTATCTTTAATTCTGAATGGCAAACCCCTTCTTTTCCT6530.13187123874146775No Hit
TTGTAGGCCCTCTCACTGTAAATGAAAAAAGAAGACTGAAATTAATTATG6360.12843814370531928No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAATTCTGAATGGCAAACCCCTT6320.12763035663799022No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6200.12520699543600305No Hit
GAAATTAATTATGCCTGCTAGGTTTTATCCTAACAGCACAAAATATTTGC6080.12278363423401592No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC6020.12157195363302234No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA5870.11854275213053839No Hit
GTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAAC5860.11834080536370613No Hit
GTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCAT5810.11733107152954482No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTGCCATTCAGAAT5790.11692717799588027No Hit
CTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCTAAAAC5720.11551355062805445No Hit
CCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA5670.11450381679389314No Hit
GGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAA5530.11167656205824145No Hit
GGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTT5410.10925320085625428No Hit
AATTATGCCTGCTAGGTTTTATCCTAACAGCACAAAATATTTGCCTTTAG5290.10682983965426714No Hit
ATCTTTAATTCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACA5280.10662789288743486No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5260.10622399935377035No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC5160.10420453168544773No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5120.10339674461811867No Hit
GCACAAAATATTTGCCTTTAGACAAAGGGATTAAACCTTATTATCCTGAT5100.10299285108445413No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC5070.10238701078395734No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5050.10198311725029283No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACACTCAACCCTGTTCCAA5040.10178117048346055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)