FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317171
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17380.5479693919053129No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC15510.48901065986486786No Hit
GCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA13860.4369882492409457No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT11980.377714229863386No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT11870.3742460691551245No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11530.3635262996932254No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10450.32947526728484006No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC10340.3260071065765786No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACA10240.3228542332054318No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10030.3162331991260235No Hit
GTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAATACGTTGACAGACT9200.29006435014550513No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC8170.2575897544226931No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT7800.24592412294944999No Hit
CTACAAACCGTGCCAACAAATCCGCCTCCTGCCTCTACCAAACGCCAGTC7800.24592412294944999No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCATGGTCCCGTG7350.23173619277928942No Hit
CTCTATGGCAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7330.23110561810506003No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7130.22479987136276644No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6530.20588263113588567No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6340.19989217173070678No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6310.19894630971936275No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6270.19768516037090403No Hit
ATGTATATTTGCGGGAAAGAACAACAGAGTTATCTGTCCCGAGAATGTTT6120.19295585031418383No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT5930.18696539090900494No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5770.18192079351517004No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5690.17939849481825262No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5600.1765609087842205No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5480.17277746073884434No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC5350.1686787253563535No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5340.16836343801923884No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5320.16773286334500948No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAAAGAACAACAGAGT5280.16647171399655075No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5250.16552585198520672No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5250.16552585198520672No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5210.164264702636748No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTCGTAGGCCCACT5170.1630035532882893No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5170.1630035532882893No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5110.1611118292656012No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5080.16016596725425716No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5080.16016596725425716No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAGCCCTGTTCCGA5020.15827424323156908No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAAT4990.15732838122022505No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4980.15701309388311036No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGATTTCCACTGCGCC4910.1548060825233076No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4900.15449079518619294No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4830.15228378382639018No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC4820.1519684964892755No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAATGTCTGGT4800.15133792181504616No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4720.1488156231181287No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGA4660.14692389909544062No Hit
CAGTAAGCCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4640.1462933244212113No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAATGTCTGGTTT4640.1462933244212113No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4630.1459780370840966No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4620.1456627497469819No Hit
GTGTAAACAATACATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4620.1456627497469819No Hit
GCACGGGACCATGCAGGACCTGCACGACTCCTGCTCAAGGAACCTCTATG4520.14250987637583512No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4490.14156401436449106No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4470.14093343969026173No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4460.14061815235314704No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4400.13872642833045898No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4400.13872642833045898No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4350.13714999164488556No Hit
GTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG4340.1368347043077709No Hit
GTATATTTGCGGGAAAGAACAACAGAGTTATCTGTCCCGAGAATGTTTGC4310.13588884229642686No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCG4300.13557355495931217No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4260.13431240561085345No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG4190.1321053942510507No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4110.12958309555413328No Hit
GCCTACCCCGCTGTCTCCACCTTTGAGAGACACTCATCCTCAGGCGCAGT4060.12800665886855986No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT4050.12769137153144516No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4010.12643022218298647No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC4000.12611493484587177No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT3990.1257996475087571No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA3990.1257996475087571No Hit
CTCTAAAGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA3940.12422321082318372No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCATGGTCCC3920.12359263614895434No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3920.12359263614895434No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3890.1226467741376103No Hit
TCCTAATACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGT3880.12233148680049565No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG3880.12233148680049565No Hit
GCATGGTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGG3870.12201619946338095No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATACTGGATCTTC3860.12170091212626627No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG3850.12138562478915159No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3800.1198091881035782No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA3760.11854803875511948No Hit
GGTATATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTC3750.11823275141800481No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3740.11791746408089013No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA3740.11791746408089013No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT3730.11760217674377543No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTATTCAAGCCTCCAAGCTGTGC3720.11728688940666075No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT3700.1166563147324314No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3670.11571045272108736No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3670.11571045272108736No Hit
CACTCATCCTCAGGCGCAGTGGAAATCCACAACCTTCCACCAAACTCTGC3650.11507987804685801No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3610.11381872869839929No Hit
GTCTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG3600.11350344136128461No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAGTCTAAGCAGGCTTTCACTTT3590.11318815402416992No Hit
GTATGCATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3590.11318815402416992No Hit
ACATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTG3550.11192700467571122No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAATCTAGTCTCCTCC3510.1106658553272525No Hit
TAACCACATTGTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAATACG3510.1106658553272525No Hit
CCTAATACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTC3490.11003528065302312No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA3470.10940470597879377No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3470.10940470597879377No Hit
GCTTTAGAGTCTCCTGAGCATTGCTCACCTCATCATACTGCACTCAGGCA3450.10877413130456443No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC3430.10814355663033505No Hit
CCCCAAAAGGCCTCCGTGAGGTGGGGTGAATCCCAGCCCGAATGCTCCAG3420.10782826929322037No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAGTCTAAGC3410.1075129819561057No Hit
GTTTGGTGGAAGGTTGTGGATTTCCACTGCGCCTGAGGATGAGTGTCTCT3390.10688240728187633No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCTTTAGAGTCTCCTGA3370.10625183260764698No Hit
ACTACAAACCGTGCCAACAAATCCGCCTCCTGCCTCTACCAAACGCCAGT3370.10625183260764698No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG3360.1059365452705323No Hit
ATATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACC3320.10467539592207359No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGATCATTGCCAC3300.10404482124784423No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT3300.10404482124784423No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3300.10404482124784423No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3290.10372953391072953No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT3270.10309895923650018No Hit
GGGCTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT3260.1027836718993855No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGAATCTTTCCACCA3260.1027836718993855No Hit
ATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATAC3260.1027836718993855No Hit
GAGTTATCTGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA3240.10215309722515614No Hit
GCTCCTACCTTGTTGGCGTCTGGCCAGGTGTCCTTGTTGGGATTGAAGTC3220.1015225225509268No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGATCAT3210.10120723521381211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)