FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317171
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT17280.5448165185341661No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17200.5422942198372487No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC14830.4675711209410696No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12780.40293721683256034No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT12570.39631618275315206No Hit
GCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA11990.3780295172005007No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG10460.3297905546219547No Hit
GTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAATACGTTGACAGACT9080.28628090210012896No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8640.27240825926708306No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8440.26610251252478945No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8410.26515665051344545No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC8250.26011205311961055No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT7880.24844642164636743No Hit
CTACAAACCGTGCCAACAAATCCGCCTCCTGCCTCTACCAAACGCCAGTC7750.2443476862638766No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACA6930.21849412462047285No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC6470.20399090711319762No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6430.2027297577647389No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT6390.20146860841628017No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6150.1939017123255279No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6100.19232527563995447No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCATGGTCCCGTG5970.18822654025746363No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5770.18192079351517004No Hit
ATGTATATTTGCGGGAAAGAACAACAGAGTTATCTGTCCCGAGAATGTTT5750.18129021884094068No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5430.17120102405327095No Hit
GGGTAAAGGTTCATGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5390.16993987470481223No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5390.16993987470481223No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5350.1686787253563535No Hit
GCATGGTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGG5170.1630035532882893No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5140.16205769127694525No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5110.1611118292656012No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5080.16016596725425716No Hit
GTGTAAACAATACATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5080.16016596725425716No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5000.15764366855733974No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTCGTAGGCCCACT4970.1566978065459957No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4960.15638251920888102No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAGCCCTGTTCCGA4960.15638251920888102No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCGAAACCCAAAAGACCCACAAT4940.15575194453465163No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4890.15417550784907827No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAAAGAACAACAGAGT4880.15386022051196357No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC4870.1535449331748489No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4800.15133792181504616No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAATGTCTGGT4770.1503920598037021No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT4770.1503920598037021No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4760.15007677246658743No Hit
GGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCCGACGAAGGTCTCAATCG4740.14944619779235807No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4720.1488156231181287No Hit
GCCTACCCCGCTGTCTCCACCTTTGAGAGACACTCATCCTCAGGCGCAGT4720.1488156231181287No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4700.14818504844389935No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4690.14786976110678468No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4620.1456627497469819No Hit
GTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4620.1456627497469819No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGA4600.14503217507275257No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGATTTCCACTGCGCC4520.14250987637583512No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC4480.1412487270273764No Hit
CACTCATCCTCAGGCGCAGTGGAAATCCACAACCTTCCACCAAACTCTGC4470.14093343969026173No Hit
GTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTG4410.13904171566757365No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAATGTCTGGTTT4410.13904171566757365No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4320.13620412963354153No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4310.13588884229642686No Hit
CAGTAAGCCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4300.13557355495931217No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4270.13462769294796814No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4260.13431240561085345No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4240.1336818309366241No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4200.1324206815881654No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4160.13115953223970667No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4150.13084424490259197No Hit
GTATATTTGCGGGAAAGAACAACAGAGTTATCTGTCCCGAGAATGTTTGC4130.1302136702283626No Hit
GCACGGGACCATGCAGGACCTGCACGACTCCTGCTCAAGGAACCTCTATG4130.1302136702283626No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4110.12958309555413328No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATACTGGATCTTC4080.12863723354278922No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4050.12769137153144516No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTC4020.12674550952010114No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4020.12674550952010114No Hit
CAAATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC4010.12643022218298647No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC3960.12485378549741306No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC3960.12485378549741306No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA3950.12453849816029838No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCG3920.12359263614895434No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3900.12296206147472499No Hit
GGTATATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTC3880.12233148680049565No Hit
GTATGCATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3850.12138562478915159No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3790.1194939007664635No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG3730.11760217674377543No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCCTGCATGGTCCC3720.11728688940666075No Hit
GTCTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCTTCTCGGGGCCG3690.11634102739531671No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG3690.11634102739531671No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAGTCTAAGCAGGCTTTCACTTT3650.11507987804685801No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTATTCAAGCCTCCAAGCTGTGC3620.11413401603551396No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3610.11381872869839929No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGATCATTAATCTAGTCTCCTCC3560.11224229201282589No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3560.11224229201282589No Hit
CCCCAAAAGGCCTCCGTGAGGTGGGGTGAATCCCAGCCCGAATGCTCCAG3560.11224229201282589No Hit
TCCTAATACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGT3510.1106658553272525No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGATCATTGCCAC3490.11003528065302312No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3460.10908941864167909No Hit
GGGCTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT3440.10845884396744973No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3440.10845884396744973No Hit
GAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC3430.10814355663033505No Hit
ACATGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTG3390.10688240728187633No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT3390.10688240728187633No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3350.10562125793341763No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCTTTAGAGTCTCCTGA3350.10562125793341763No Hit
GTTTGGTGGAAGGTTGTGGATTTCCACTGCGCCTGAGGATGAGTGTCTCT3340.10530597059630294No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG3310.10436010858495891No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAGTCTAAGC3300.10404482124784423No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGATCAT3290.10372953391072953No Hit
ACTACAAACCGTGCCAACAAATCCGCCTCCTGCCTCTACCAAACGCCAGT3290.10372953391072953No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3280.10341424657361487No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT3260.1027836718993855No Hit
CCTAATACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTC3240.10215309722515614No Hit
GACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG3210.10120723521381211No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA3190.10057666053958274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)