FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406701
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCGGGTTTGGAAGTAATGATTAACTAGA25840.6353561953376067No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC18670.4590596039842538No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18340.45094553492614964No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG16390.40299876321917083No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14230.3498884930206712No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC13270.3262839284880047No Hit
GGTTTGTAGTGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT11810.2903853199279077No Hit
CCTCCAGCTTATAGACCATCAAATGCCCCTATCTTATCAACACTTCCGGA11800.2901394390473591No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11490.2825171317503522No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGATCACCATATT11010.27071484948401897No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG10340.25424083048726215No Hit
GTTTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC9710.23875033501269974No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC9320.22916098067130397No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9230.2269480527463665No Hit
CTCTAAAACTGTTCCTTTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9050.22252219689649153No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8890.21858810280771376No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG8590.2112116763912555No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8140.2001470367665681No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8000.19670470443888755No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7950.1954753000361445No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7920.19473765739449866No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGATCACCATA7780.19129532506681812No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7720.1898200397835265No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7600.18686946921694314No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7480.18391889865035985No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC7430.1826894942476168No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT7300.17949304280048486No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCGGGT7160.17605071047280435No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7010.1723624972645752No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC6980.1716248546229294No Hit
GGGTAAAGGTTCAGGTATTGATTACGCAGAAAGGCCTTGTAAGTTGGCGA6960.17113309286183215No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT6920.1701495693396377No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6670.16400254732592248No Hit
CTCATATGGGCCTCAAATTCCGGCAACTATTGTGGTTTCACATTTCTTGT6630.16301902380372804No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCCAAT6610.16252726204263082No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT6520.16031433411769336No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATT6490.15957669147604753No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6480.1593308105954989No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6320.15539671650672116No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6290.15465907386507532No Hit
ATCAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGC6250.15367555034288088No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC6190.15220026505958922No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAAA6160.1514626224179434No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC6140.1509708606568462No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6090.14974145625410315No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6050.14875793273190868No Hit
GTGTAAATGTATATTGGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA6010.14777440920971427No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5980.14703676656806844No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCGGGTTT5860.14408619600148512No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG5800.14261091071819346No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5750.1413815063154504No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5630.1384309357488671No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG5590.13744741222667264No Hit
GAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA5570.13695565046557545No Hit
CCTTATGAGTCCAAGGAATACTAACATTGGGATCCCCGAGATTGAGATCT5450.13400507989899213No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC5400.13277567549624908No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATCAAGGAATGT5370.13203803285460325No Hit
CAGACACATCCAGCGATAACCAGGACAAGTTGGAGGACAGCAGGTTGGTG5340.13130039021295742No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5220.12834981964637412No Hit
GTACAGTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA5190.1276121770047283No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5190.1276121770047283No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5160.12687453436308246No Hit
GGTATTGATTACGCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCC5100.1253992490797908No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5100.1253992490797908No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCC5060.12441572555759636No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4950.12171103587156167No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG4940.12146515499101307No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4930.12121927411046445No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC4900.12048163146881861No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4870.11974398882717278No Hit
ATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGAACTAATGACTCTA4850.11925222706607556No Hit
TCATTAATCTATGCTCCTCCCCCAACTCCTCCCAGTCTTTGAACAAACAG4840.11900634618552697No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4840.11900634618552697No Hit
ACCATATTCTTGGGAACAAAAGCTACAGCATGGGGCAGAATCTTTCCACC4800.11802282266333251No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4800.11802282266333251No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT4770.11728518002168668No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA4720.11605577561894363No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTCATATGGGC4690.11531813297729782No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4670.11482637121620061No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT4660.11458049033565199No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4620.11359696681345756No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4620.11359696681345756No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4620.11359696681345756No Hit
CTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTC4590.11285932417181173No Hit
GAATACTAACATTGGGATCCCCGAGATTGAGATCTTCCGCGACGTGGCGA4570.11236756241071451No Hit
GTGTAAATAATGTCGGGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT4560.11212168153016591No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4560.11212168153016591No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCCAATATACATTTACA4510.11089227712742286No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG4510.11089227712742286No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4490.11040051536632563No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGATTACGC4460.10966287272467981No Hit
CATGTATTCAGTCGAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4460.10966287272467981No Hit
ATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCA4450.1094169918441312No Hit
ATATGGGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG4450.1094169918441312No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG4430.10892523008303398No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4410.10843346832193676No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGAACTAATGA4390.10794170656083953No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4390.10794170656083953No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4360.10720406391919371No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT4330.10646642127754788No Hit
GCTTTAGGGCATGGATCTTGATCCTTATAAAGAATTTGGAGCTTCTGTGG4320.10622054039699927No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA4310.10597465951645066No Hit
AAACAACACATAGCGCCTCATTTTGTGGATCACCATATTCTTGGGAACAA4310.10597465951645066No Hit
GTCATTGGCTGTTATGGGTCATTGCCACAAGATCACATCATACAAAAAAT4300.10572877863590205No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACTG4270.10499113599425622No Hit
GTCCAAGGAATACTAACATTGGGATCCCCGAGATTGAGATCTTCCGCGAC4230.10400761247206178No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACC4220.10376173159151317No Hit
ACCCATAACAGCCAATGACATAGCCCATGAAATGTAAAGAGTAACCCCAT4210.10351585071096457No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGCTGTTATGGGTCAT4210.10351585071096457No Hit
CCCCTATCTTATCAACACTTCCGGAGACTTGTGTTATTAGACAACGAGGC4190.10302408894986735No Hit
TCTATGCTCCTCCCCCAACTCCTCCCAGTCTTTGAACAAACAGTCTTTGA4190.10302408894986735No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4140.1017946845471243No Hit
GTCTTGGTGTAAATGTATATTGGGAAAAGAAGGTGTTTTCCAATGAGGGT4140.1017946845471243No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4120.10130292278602707No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4120.10130292278602707No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4110.10105704190547847No Hit
GGGTAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT4110.10105704190547847No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4090.10056528014438125No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4070.10007351838328404No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAA4070.10007351838328404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)