FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406701
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCGGGTTTGGAAGTAATGATTAACTAGA21270.5229886329268922No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG18550.4561090334176705No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC17670.43447151592939287No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16160.3973435029665528No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC14140.3476755650957337No Hit
CCTCCAGCTTATAGACCATCAAATGCCCCTATCTTATCAACACTTCCGGA14000.3442332327680532No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13260.3260380476074561No Hit
GGTTTGTAGTGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT11830.29087708168900495No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11530.28350065527254664No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG10930.2687478024396301No Hit
GTTTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC10110.24858557023464414No Hit
CTCTAAAACTGTTCCTTTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9940.24440559526531777No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGATCACCATATT9330.22940686155185258No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG9230.2269480527463665No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8500.208998748466318No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8400.2065399396608319No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCCAAT7820.19227884858901256No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7730.19006592066407507No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7700.18932827802242924No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGATCACCATA7650.1880988736196862No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT7500.18441066041145707No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT7470.18367301776981124No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7340.18047656632267933No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC7180.17654247223390157No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7160.17605071047280435No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT7050.17334602078676964No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6910.1699036884590891No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCGGGT6870.16892016493689466No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG6790.1669531178925058No Hit
GGGTAAAGGTTCAGGTATTGATTACGCAGAAAGGCCTTGTAAGTTGGCGA6770.16646135613140858No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG6650.16351078556482526No Hit
GTGTAAATGTATATTGGGAAAAGAAGGTGTTTTCCAATGAGGGTTAAAGA6400.15736376355111004No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAAAA6320.15539671650672116No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6320.15539671650672116No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATT6300.15490495474562394No Hit
ATCAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGC6250.15367555034288088No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6110.15023321801520037No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC6070.14924969449300593No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC6060.14900381361245732No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5960.14654500480697122No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG5940.146053243045874No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC5940.146053243045874No Hit
CTCATATGGGCCTCAAATTCCGGCAACTATTGTGGTTTCACATTTCTTGT5850.1438403151209365No Hit
GTCTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATAC5830.1433485533598393No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5800.14261091071819346No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5770.14187326807654763No Hit
GAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA5770.14187326807654763No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5740.1411356254349018No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5670.13941445927106152No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAAAAAATCAAGGAATGT5630.1384309357488671No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5630.1384309357488671No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG5620.1381850548683185No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5380.1322839137351519No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5350.13154627109350606No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCC5320.13080862845186023No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5270.12957922404911718No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG5250.12908746228801996No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5200.1278580578852769No Hit
CTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCTTCTTTTC5150.12662865348253385No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG5120.12589101084088802No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATG5060.12441572555759636No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCGGGTTT5030.12367808291595055No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC5000.12294044027430472No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5000.12294044027430472No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4980.1224486785132075No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4960.12195691675211026No Hit
ATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCA4960.12195691675211026No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4950.12171103587156167No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4850.11925222706607556No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA4820.11851458442442973No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4800.11802282266333251No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTCATATGGGC4790.11777694178278392No Hit
GTACAGTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA4770.11728518002168668No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4750.11679341826058949No Hit
GGTATTGATTACGCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCC4700.11556401385784644No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4700.11556401385784644No Hit
GCCTAATCATCTTTTGTTCCATGTCCTACTGTTCAAGCCTCCAAGCTGTG4690.11531813297729782No Hit
GTCCAAGGAATACTAACATTGGGATCCCCGAGATTGAGATCTTCCGCGAC4670.11482637121620061No Hit
GCTTTAGGGCATGGATCTTGATCCTTATAAAGAATTTGGAGCTTCTGTGG4660.11458049033565199No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4660.11458049033565199No Hit
ATATGGGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG4620.11359696681345756No Hit
GTCTTTAACCCTCATTGGAAAACACCTTCTTTTCCCAATATACATTTACA4610.11335108593290893No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4600.11310520505236034No Hit
CCTTATGAGTCCAAGGAATACTAACATTGGGATCCCCGAGATTGAGATCT4600.11310520505236034No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACC4590.11285932417181173No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT4530.11138403888852005No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4500.11064639624687424No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4470.10990875360522841No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4460.10966287272467981No Hit
GTCTTGGTGTAAATGTATATTGGGAAAAGAAGGTGTTTTCCAATGAGGGT4440.10917111096358258No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA4430.10892523008303398No Hit
TCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC4430.10892523008303398No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA4420.10867934920248536No Hit
GTATGCATGTATTCAGTCGAAGCAGGCTTTTACTTTCTCGCCAACTTACA4360.10720406391919371No Hit
ACCATATTCTTGGGAACAAAAGCTACAGCATGGGGCAGAATCTTTCCACC4330.10646642127754788No Hit
CATGTATTCAGTCGAAGCAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4280.10523701687480483No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGTCG4270.10499113599425622No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4250.104499374233159No Hit
CCCCTATCTTATCAACACTTCCGGAGACTTGTGTTATTAGACAACGAGGC4240.1042534933526104No Hit
GCTTTAAAGCCCTTGTATGCATGTATTCAGTCGAAGCAGGCTTTTACTTT4230.10400761247206178No Hit
GAATACTAACATTGGGATCCCCGAGATTGAGATCTTCCGCGACGTGGCGA4180.10277820806931874No Hit
CTTTTGTTCCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4160.10228644630822152No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4160.10228644630822152No Hit
GTCATTGGCTGTTATGGGTCATTGCCACAAGATCACATCATACAAAAAAT4150.10204056542767292No Hit
GTCTAATAACACAAGTCTCCGGAAGTGTTGATAAGATAGGGGCATTTGAT4140.1017946845471243No Hit
CTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCTGAACATG4120.10130292278602707No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4120.10130292278602707No Hit
CAGACACATCCAGCGATAACCAGGACAAGTTGGAGGACAGCAGGTTGGTG4100.10081116102492987No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4090.10056528014438125No Hit
ATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGAACTAATGACTCTA4080.10031939926383264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)