FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472510
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC25940.5489830903049671No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT17620.3729021608008296No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT17550.37142071067279003No Hit
CCTCCACCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16030.3392520793210726No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15570.32951683562252654No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA15270.3231677636452138No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAAACACCGTG14980.3170303274004783No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT13190.2791475312691795No Hit
CATATAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT13110.2774544454085628No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGTCCTACAATACGTTGACAGACT12520.26496793718651457No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC12420.2628515798607437No Hit
GTCTTAGAGTACTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCACC11690.24740217138261625No Hit
TCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA11130.2355505703582993No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT10790.2283549554506783No Hit
CGGTATTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC10020.21205900404224248No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9830.20803792512327784No Hit
GTCACTTATGTCAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTG9810.20761465365812365No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC9520.20147721741338806No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC9510.201265581680811No Hit
TACATGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGATAC9510.201265581680811No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCCG9300.19682123129669216No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9280.19639795983153796No Hit
CCTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAA9210.19491650970349833No Hit
CTCTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGT9150.1936466953080358No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC9140.19343505957545873No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTGGATGGGCGTTC9110.19280015237772746No Hit
GAAACACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC8960.18962561638907113No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG8930.18899070919133987No Hit
CTCTAAGACTGTCTCTCTCCCAAAAGTAAGACAAGAAATGTGAAACCACA8810.1864510804004148No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT8750.18518126600495227No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8660.18327654441175847No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA8520.18031364415567924No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8110.17163657912001862No Hit
GAGAAACACGCACCCGCAAGCCATGCAGTGGAACTCCACAACCTTCCACC7990.16909695032909358No Hit
GGTTTATATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA7920.16761550020105395No Hit
ATGTAAGACCTTGGGCAAGACTGGATGGGCGTTCACGGTGGTCTCCATGC7910.16740386446847685No Hit
CTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTT7910.16740386446847685No Hit
AAGATGGTGTTTTCCAATGAGGGTTAAAGATAGGTACAGTAGAAGAGTAA7840.16592241434043722No Hit
CTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAAC7780.16465259994497472No Hit
ATCAACTGCAGTCTTCTTTTCTCATTGACTGTGAGTGGGCCTACAAATTG7700.162959514084358No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA7590.16063152102601003No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT7440.1574569850373537No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7400.15661044210704536No Hit
ACCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTA7330.15512899197900576No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7270.1538591775835432No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC7260.15364754185096613No Hit
GAGTACTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCACCTTATAG7250.15343590611838903No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7240.15322427038581193No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7230.15301263465323486No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6900.14602865547819094No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6860.14518211254788257No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6820.14433556961757424No Hit
CCCCAGAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCCG6780.14348902668726587No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGTCCTACAAT6750.1428541194895346No Hit
CTCTGTGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6720.14221921229180334No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6690.14158430509407208No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6560.13883304057056994No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG6550.13862140483799285No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACT6380.13502359738418235No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6320.13375378298871982No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGTTAAAGA6280.13290724005841145No Hit
GATCCAACAGCAAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGG6260.13248396859325728No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6220.13163742566294892No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6180.13079088273264058No Hit
CATAACTGACTACTAGGTCCCTTGCTGTTTGATCTTCCAAATTACCACCC6160.1303676112674864No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTATCCTTCAGTACG6150.13015597553490932No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACA6130.12973270406975515No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6110.12930943260460098No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAGCATTCTTTGATTTTCTGT6080.1286745254068697No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6020.12740471101140716No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6000.126981439546253No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT5990.1267698038136759No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT5960.12613489661594462No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5930.12549998941821336No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA5920.12528835368563626No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC5900.1248650822204821No Hit
GTCTCCTTTTCTCATTGACTGTGAGTGGGCCTACAAATTGTTCACATTTT5840.12359526782501959No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5790.12253708916213414No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5770.12211381769697996No Hit
GTCTTTAAACAAACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACA5750.12169054623182578No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGTCCTACAATACG5700.12063236756894033No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCCGTCCCGATAATGTTT5690.12042073183636325No Hit
CTTCTACTGTACCTATCTTTAACCCTCATTGGAAAACACCATCTTTTCCT5680.12020909610378616No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAAC5680.12020909610378616No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5680.12020909610378616No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG5620.11893928170832364No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5620.11893928170832364No Hit
CCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAACATTGAGATTC5570.1178811030454382No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5570.1178811030454382No Hit
GAGATACAGTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACT5490.11618801718482148No Hit
CCAAATACTCTAAGACTGTATCTCTCCCAAAAGTAAGACAAGAAATGTGA5480.1159763814522444No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5470.1157647457196673No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5440.11512983852193603No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5430.11491820278935895No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC5380.11386002412647352No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC5320.11259020973101098No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5290.11195530253327972No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5250.11110875960297137No Hit
CTGTCTCTCTCCCAAAAGTAAGACAAGAAATGTGAAACCACAATAGTTGC5190.10983894520750882No Hit
GTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCTTCCTGACTGGCG5180.10962730947493174No Hit
CTATAAGGTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAGTACTC5170.10941567374235467No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5160.10920403800977758No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG5120.10835749507946922No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5100.10793422361431504No Hit
GTACAGTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA5090.10772258788173796No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5070.10729931641658377No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5060.10708768068400669No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5050.1068760449514296No Hit
TCACCATACTGCACTCAGGCAAGCAATTGTGTGCTGGGGGGAATTAATGA5000.10581786628854416No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC4990.10560623055596706No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4960.1049713233582358No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC4960.1049713233582358No Hit
ATACTGCACTCAGGCAAGCAATTGTGTGCTGGGGGGAATTAATGACTCTA4920.10412478042792746No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA4910.10391314469535037No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4890.10348987323019619No Hit
GTGTTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG4800.1015851516370024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)