FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472510
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC21560.45628663943620246No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT17480.3699392605447504No Hit
CCTCCACCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16850.3566062093923938No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAAACACCGTG16540.3500455016825041No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAGTT15360.32507248523840765No Hit
ATCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT13970.2956551184101924No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA13860.2933271253518444No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13630.2884595035025714No Hit
CATATAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT12880.27258682355928976No Hit
GTCTTAGAGTACTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCACC12020.2543861505576602No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGTCCTACAATACGTTGACAGACT11940.25269306469704345No Hit
CTACAAACCTTGCCAACAAATCCGCCTCCTGCCTCCACCAATCGCCAGTC11340.23999492074241813No Hit
TCATCTTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT10950.2317411271719117No Hit
TCCACAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA10390.21988952614759474No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10060.21290554697255085No Hit
GTCACTTATGTCAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTG9010.1906837950519566No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT8730.1847579945397981No Hit
GAAACACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC8470.1792554654927938No Hit
CTCTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGT8340.17650420096929165No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8300.1756576580389833No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC8230.17417620791094368No Hit
CTCTAAGACTGTCTCTCTCCCAAAAGTAAGACAAGAAATGTGAAACCACA8200.17354130071321242No Hit
CCTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAA8180.17311802924805825No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC8170.17290639351548115No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCCG8140.17227148631774988No Hit
CGGTATTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC8120.17184821485259572No Hit
TACATGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGATAC8080.17100167192228735No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7780.16465259994497472No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7680.16253624261920382No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7680.16253624261920382No Hit
AAGATGGTGTTTTCCAATGAGGGTTAAAGATAGGTACAGTAGAAGAGTAA7390.15639880637446826No Hit
GAGAAACACGCACCCGCAAGCCATGCAGTGGAACTCCACAACCTTCCACC7350.1555522634441599No Hit
CTTATATAATATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTGGTTT7290.1542824490486974No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTGGATGGGCGTTC7280.1540708133161203No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA7260.15364754185096613No Hit
GTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT7160.15153118452519523No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7050.14920319146684727No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7010.1483566485365389No Hit
GAGTACTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCACCTTATAG6970.14751010560623057No Hit
ATCAACTGCAGTCTTCTTTTCTCATTGACTGTGAGTGGGCCTACAAATTG6930.1466635626759222No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6900.14602865547819094No Hit
CTTCTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAAC6860.14518211254788257No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6840.1447588410827284No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6830.14454720535015134No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6660.1409493978963408No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC6640.14052612643118664No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6560.13883304057056994No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6550.13862140483799285No Hit
GGTTTATATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA6540.13840976910541578No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGTCCTACAAT6470.13692831897737615No Hit
CCCCAGAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCCG6420.13587014031449068No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6320.13375378298871982No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACA6320.13375378298871982No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA6290.13311887579098855No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6230.13184906139552602No Hit
ACCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTA6150.13015597553490932No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6150.13015597553490932No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6120.12952106833717805No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6110.12930943260460098No Hit
GATCCAACAGCAAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGG6070.12846288967429262No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6000.126981439546253No Hit
CCAAATACTCTAAGACTGTATCTCTCCCAAAAGTAAGACAAGAAATGTGA5970.12634653234852172No Hit
AATATACATCGTTTCCATGGCTGCTCGGCTGTACTGCCAACTGGATCCTG5930.12549998941821336No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG5890.12465344648790502No Hit
CTCTGTGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5870.12423017502275085No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5860.12401853929017376No Hit
GTGTAAATGTATATTAGGAAAAGATGGTGTTTTCCAATGAGGGTTAAAGA5850.12380690355759667No Hit
CTATAAGGTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAGTACTC5820.12317199635986541No Hit
ATGTAAGACCTTGGGCAAGACTGGATGGGCGTTCACGGTGGTCTCCATGC5820.12317199635986541No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG5600.11851601024316946No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5570.1178811030454382No Hit
CTTCTACTGTACCTATCTTTAACCCTCATTGGAAAACACCATCTTTTCCT5550.11745783158028401No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5480.1159763814522444No Hit
GTCTCCTTTTCTCATTGACTGTGAGTGGGCCTACAAATTGTTCACATTTT5470.1157647457196673No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5460.11555310998709022No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATACTACAAACCTTGCCAAC5430.11491820278935895No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAGCATTCTTTGATTTTCTGT5410.1144949313242048No Hit
GTCTTTAAACAAACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACA5380.11386002412647352No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5260.11132039533554845No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTATCCTTCAGTACG5240.11089712387039427No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5240.11089712387039427No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG5190.10983894520750882No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5150.10899240227720049No Hit
GCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC5100.10793422361431504No Hit
CTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCAC5100.10793422361431504No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5070.10729931641658377No Hit
CTGTCTCTCTCCCAAAAGTAAGACAAGAAATGTGAAACCACAATAGTTGC5060.10708768068400669No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5060.10708768068400669No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGTCCTACAATACG5040.10666440921885251No Hit
CATAACTGACTACTAGGTCCCTTGCTGTTTGATCTTCCAAATTACCACCC5040.10666440921885251No Hit
ATACTGCACTCAGGCAAGCAATTGTGTGCTGGGGGGAATTAATGACTCTA5030.10645277348627542No Hit
GAGATACAGTCTTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACT5020.10624113775369833No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4980.10539459482338998No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC4970.10518295909081289No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG4950.10475968762565871No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4950.10475968762565871No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4930.10433641616050456No Hit
GTACAGTAGAAGAGTAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAA4880.10327823749761911No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACT4840.10243169456731074No Hit
GGGTTACTCTTTACATTTCATGGGATATGTCATTGGAGGTTATGGATCAT4800.1015851516370024No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTGTCCGTCCCGATAATGTTT4800.1015851516370024No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4770.10095024443927113No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC4760.10073860870669403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)